| Recombinant human bone morphogenetic protein-7 enhances fracture healing in an ischemic environment. | |
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MedLine Citation:
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PMID: 19918910 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ischemia predisposes orthopedic trauma patients to delayed fracture healing or nonunion. The goal of this study was to test the ability of bone morphogenetic protein 7 (BMP7) to stimulate fracture repair in an ischemic environment. Ischemic fractures were generated in male adult mice by resecting the femoral artery prior to the creation of a nonstabilized tibia fracture. Recombinant human BMP7 (rhBMP7, 50 microg) was injected into the fracture site immediately after surgery. At 7 days after injury, more tissue vascularization was observed in rhBMP7 treated fractures. Histomorphometric analyses revealed that rhBMP7 induced more cartilage at day 7, more callus and bone at days 14 and 28, and more adipose tissue and fibrous tissue at days 7, 14, and 28 compared to controls (n=5/group/time). At day 28, all fractures treated with rhBMP7 (50 microg, n=5) healed, whereas only three of five control fractures exhibited slight bony bridging. In addition, we found that rhBMP7 (both 10 and 50 microg) significantly increased the amount of cartilage compared to controls in stabilized fractures, confirming its chondrogenic effect. Lastly, using bone marrow transplantation, we determined that no donor-derived osteocytes or chondrocytes were present in rhBMP7-treated fractures, suggesting rhBMP7 did not recruit mesenchymal stem cells from the bone marrow to the fracture site. In conclusion, our results indicate that rhBMP7 is a promising treatment for fractures with severely disrupted blood supply. |
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Authors:
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Chuanyong Lu; Zhiqing Xing; Yan-yiu Yu; Celine Colnot; Theodore Miclau; Ralph S Marcucio |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of orthopaedic research : official publication of the Orthopaedic Research Society Volume: 28 ISSN: 1554-527X ISO Abbreviation: J. Orthop. Res. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-03-25 Completed Date: 2010-04-13 Revised Date: 2011-07-28 |
Medline Journal Info:
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Nlm Unique ID: 8404726 Medline TA: J Orthop Res Country: United States |
Other Details:
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Languages: eng Pagination: 687-96 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2009 Orthopaedic Research Society. |
Affiliation:
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Department of Orthopaedic Surgery, San Francisco General Hospital, University of California at San Francisco, 1001 Potrero Avenue, San Francisco, California 94110, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bone Marrow Transplantation Bone Morphogenetic Protein 7 / pharmacology* Chondrogenesis / drug effects, physiology Femoral Artery Fracture Healing / drug effects* Ischemia / complications* Male Mice Mice, Inbred Strains Neovascularization, Physiologic / drug effects, physiology Osteogenesis / drug effects, physiology Recombinant Proteins / pharmacology* Severity of Illness Index Tibia / blood supply, injuries, physiology Tibial Fractures / complications*, drug therapy* Transplantation Chimera |
| Grant Support | |
ID/Acronym/Agency:
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K08 AR002164-05/AR/NIAMS NIH HHS; R01 AR053645-01/AR/NIAMS NIH HHS; R01-AR053645-01/AR/NIAMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Bone Morphogenetic Protein 7; 0/Recombinant Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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