Document Detail


Recombinant cell bioassays for endocrine disruptors: development of a stably transfected human ovarian cell line for the detection of estrogenic and anti-estrogenic chemicals.
MedLine Citation:
PMID:  10900408     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The ability of a variety of compounds to disrupt normal endocrine homeostasis, and potentially, the physiological and reproductive capacity of an organism, has gained worldwide attention in recent years. In an attempt to identify such compounds, and to examine the mechanism(s) by which they may exert their actions, we have constructed reporter plasmid vectors that contain the firefly luciferase gene under hormone-inducible control of estrogen-, androgen-, or retinoic acid-responsive DNA enhancer elements. Transient transfection of these vectors into appropriate receptor-containing cell lines revealed their ability to respond to their respective hormones with the induction of luciferase. Here, we describe development and optimization of a recombinant human ovarian carcinoma (BG-1) line, which has been stably transfected with the estrogen responsive luciferase reporter plasmid. The resulting recombinant cell line (BG1Luc4E(2)) responds to 17beta-estradiol at concentrations as low as 1 pM. The utility of BG1Luc4E(2) cells as a bioassay screening system for environmental estrogens was demonstrated by their response to known xenoestrogens, and also by the putative identification of two polychlorinated biphenyls (2,3',4, 4,'-tetrachlorobiphenyl and 2,2',3,5',6-pentachlorobiphenyl) as novel estrogenic chemicals. These cell bioassay systems have applications for rapid screening, identification, and characterization of endocrine disrupting chemicals.
Authors:
J M Rogers; M S Denison
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  In vitro & molecular toxicology     Volume:  13     ISSN:  1097-9336     ISO Abbreviation:  In Vitr Mol Toxicol     Publication Date:  2000  
Date Detail:
Created Date:  2000-08-15     Completed Date:  2000-08-15     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9808800     Medline TA:  In Vitr Mol Toxicol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  67-82     Citation Subset:  IM    
Affiliation:
Department of Environmental Toxicology, University of California, Davis 95616, USA.
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MeSH Terms
Descriptor/Qualifier:
Androgens / pharmacology
Animals
Base Sequence
Biological Assay / methods*
Dose-Response Relationship, Drug
Enhancer Elements, Genetic / genetics
Estrogen Antagonists / pharmacology
Estrogen Receptor Modulators / analysis*,  pharmacology*,  toxicity
Estrogens / analysis*,  pharmacology*,  toxicity
Female
Genes, Reporter / genetics
Humans
Molecular Sequence Data
Ovary / drug effects*,  metabolism
Polychlorinated Biphenyls / analysis,  pharmacology,  toxicity
Receptors, Estrogen / metabolism
Sensitivity and Specificity
Substrate Specificity
Tamoxifen / pharmacology
Time Factors
Transcriptional Activation / drug effects
Tretinoin / pharmacology
Tumor Cells, Cultured
Grant Support
ID/Acronym/Agency:
ES04699/ES/NIEHS NIH HHS; ES07685/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Androgens; 0/Estrogen Antagonists; 0/Estrogen Receptor Modulators; 0/Estrogens; 0/Polychlorinated Biphenyls; 0/Receptors, Estrogen; 10540-29-1/Tamoxifen; 302-79-4/Tretinoin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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