| Recombinant bovine pancreatic trypsin inhibitor protects the liver from carbon tetrachloride-induced acute injury in mice. | |
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MedLine Citation:
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PMID: 20487216 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: Toxicity caused by pharmacological and chemical substances, including carbon tetrachloride (CCl(4)), is a major pathological factor for liver injury. Therefore, strategies to prevent toxicity are needed for maintaining a healthy liver. This study was designed to determine whether recombinant bovine pancreatic trypsin inhibitor (rBPTI), a non-specific serine protease inhibitor, prevents CCl(4)-induced liver injury in mice. METHODS: Mice were treated with CCl(4) in the presence or absence of co-treatment with rBPTI. Liver sections were prepared for histopathological assessment. Liver function was evaluated by detecting serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and liver index. Liver oxidative stress and inflammation were examined by detecting the liver malondialdehyde level and glutathione and superoxide dismutase activity, and serum tumour necrosis factor-alpha level, respectively. KEY FINDINGS: CCl(4) induced hepatocyte necrosis, inflammatory cell infiltration and fatty degeneration, which were ameliorated by co-treatment with rBPTI in a concentration-dependent manner. Furthermore, rBPTI prevented CCl(4)-induced disruption of liver function. Importantly, rBPTI reduced CCl(4)-induced liver oxidative stress response and pro-inflammatory cytokine production. CONCLUSIONS: These results indicated that rBPTI exerted a protective effect on CCl(4)-induced liver injury in mice. Thus, rBPTI may have potential application for prevention of liver injury induced by metabolism of drugs and toxic substances. |
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Authors:
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Lili Yang; Wen Dong; Fang Yan; Xiubao Ren; Xishan Hao |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of pharmacy and pharmacology Volume: 62 ISSN: 2042-7158 ISO Abbreviation: J. Pharm. Pharmacol. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-05-21 Completed Date: 2010-09-10 Revised Date: 2011-09-13 |
Medline Journal Info:
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Nlm Unique ID: 0376363 Medline TA: J Pharm Pharmacol Country: England |
Other Details:
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Languages: eng Pagination: 332-8 Citation Subset: IM |
Affiliation:
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Department of Immunology, Key Laboratory of Cancer Prevention and Therapy, Tianjin Cancer Institute and Hospital, Tianjin Medical University, Tianjin, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aprotinin / biosynthesis, isolation & purification, therapeutic use* Carbon Tetrachloride / toxicity* Dose-Response Relationship, Drug Drug-Induced Liver Injury / blood, metabolism, pathology, prevention & control* Fatty Liver / chemically induced, pathology, prevention & control Female Glutathione / metabolism Inflammation / metabolism Liver / drug effects*, metabolism, pathology Male Malondialdehyde / metabolism Mice Necrosis / chemically induced, prevention & control Neutrophil Infiltration / drug effects Organ Size / drug effects Oxidative Stress / drug effects Protective Agents / isolation & purification, metabolism, therapeutic use* Random Allocation Recombinant Proteins / biosynthesis, isolation & purification, therapeutic use Severity of Illness Index Superoxide Dismutase / metabolism Tumor Necrosis Factor-alpha / blood |
| Chemical | |
Reg. No./Substance:
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0/Protective Agents; 0/Recombinant Proteins; 0/Tumor Necrosis Factor-alpha; 542-78-9/Malondialdehyde; 56-23-5/Carbon Tetrachloride; 70-18-8/Glutathione; 9087-70-1/Aprotinin; EC 1.15.1.1/Superoxide Dismutase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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