Document Detail


Recombinant adenovirus encoding the HA gene from swine H3N2 influenza virus partially protects mice from challenge with heterologous virus: A/HK/1/68 (H3N2).
MedLine Citation:
PMID:  12417948     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Immunization with recombinant adenoviral vaccine that induces potent immunity has been applied to many infectious diseases. We report here developing a recombinant adenoviral vaccine encoding the HA gene from swine H3N2 influenza virus (SIV). Two replication-defective recombinant adenoviruses were generated: (1) rAd-HA: recombinant adenovirus encoding the HA gene from swine H3N2 influenza virus, and (2) rAd-vector: a control recombinant adenovirus containing adenovirus and transfer plasmids without a foreign HA gene. Mice given rAd-HA developed high titers of neutralizing and hemagglutination inhibition antibodies to SIV in comparison to mice inoculated with rAd-vector or PBS as early as 2 weeks after immunization, and these antibodies were substantially increased in the mice given rAd-HA within the next 3 weeks following the first dose. However, these antibodies were not able to neutralize the virus, A/HK/68 (H3N2), used for challenge. Nonetheless mice immunized with one or two doses of rAd-HA were protected from lethal challenge with heterologous virus, A/HK/1/68 (H3N2). A statistically significant ( P < 0.03) difference between survival rates of rAd-HA mice vs. rAd-vector or PBS mice was observed.
Authors:
M Tang; J A Harp; R D Wesley
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of virology     Volume:  147     ISSN:  0304-8608     ISO Abbreviation:  Arch. Virol.     Publication Date:  2002 Nov 
Date Detail:
Created Date:  2002-11-05     Completed Date:  2003-01-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7506870     Medline TA:  Arch Virol     Country:  Austria    
Other Details:
Languages:  eng     Pagination:  2125-41     Citation Subset:  IM    
Affiliation:
Virus and Prion Diseases of Livestock Research Unit, National Animal Disease Center, USDA, Ames, Iowa, USA.
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MeSH Terms
Descriptor/Qualifier:
Adenoviridae / genetics,  immunology
Animals
Antibodies, Viral / biosynthesis
Defective Viruses / immunology
Female
Hemagglutination Inhibition Tests
Hemagglutinin Glycoproteins, Influenza Virus / genetics*,  immunology
Immunization
Influenza A Virus, H3N2 Subtype*
Influenza A virus / immunology*
Influenza Vaccines / immunology*
Mice
Mice, Inbred BALB C
Orthomyxoviridae Infections / prevention & control*
Recombinant Proteins / immunology
Vaccines, Synthetic / immunology*
Chemical
Reg. No./Substance:
0/Antibodies, Viral; 0/Hemagglutinin Glycoproteins, Influenza Virus; 0/Influenza Vaccines; 0/Recombinant Proteins; 0/Vaccines, Synthetic

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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