Document Detail


Recognition and uptake of free and nanoparticle-bound betalactoglobulin - a food allergen - by human monocytes.
MedLine Citation:
PMID:  21953745     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Scope: To improve our understanding of the interaction of food allergens with cells of the immune system, the endocytosis by human monocytes of bovine β-lactoglobulin (BLG) and ovomucoid (OM) - two major food allergens - and human serum albumin (HSA) was studied. Methods and results: BLG was covalently conjugated to dextran-coated magnetic nanoparticles (MNPs) without affecting its structure and immunoreactivity. BLG-conjugated MNPs were taken up by human monocytes much more efficiently than non-conjugated MNPs, allowing easy magnetic separation of cells that had adsorbed the allergen. BLG, OM, and HSA were conjugated to MNPs also labeled with a fluorescent probe. The uptake of these materials by human monocytes was monitored through flow cytometry, and compared with fluorescent MNPs and the free fluorescently labeled proteins, confirming higher uptake of the BLG-conjugated MNPs versus non-conjugated MNPs. OM but not HSA conjugation to particles enhanced uptake of the MNPs. Confocal microscopy provided direct evidence of the actual internalization of BLG-MNP conjugates into the cytoplasm. Conclusions: These results contribute to the current understanding of the interaction between food allergens and antigen-presenting cells, and demonstrate that the BLG is readily endocytosed by monocytes both as the single protein and as a conjugate.
Authors:
Mauro Marengo; Francesco Bonomi; Stefania Iametti; Eva Prinz; Rolf Hempelmann; Mette Boye; Hanne Frokiaer
Related Documents :
21967645 - Lack of significant food effect on the pharmacokinetics of ticagrelor in healthy volunt...
2726745 - Lithium evokes expression of vegetal-specific molecules in the animal blastomeres of se...
24476635 - Mediation of parental educational level on fruit and vegetable intake among schoolchild...
1760755 - Problems and practice in mass medication of beef cattle.
24534515 - Short communication: occurrence of arcobacter species in industrial dairy plants.
23493535 - Comparison of nutrition standards and other recommended procurement practices for impro...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-9-23
Journal Detail:
Title:  Molecular nutrition & food research     Volume:  -     ISSN:  1613-4133     ISO Abbreviation:  -     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-9-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101231818     Medline TA:  Mol Nutr Food Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Affiliation:
Dipartimento di Scienze Molecolari Agroalimentari, University of Milan, Milan, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Validation of a radioimmunoassay for measuring testosterone concentrations in plasma samples of the ...
Next Document:  Cardiogenic shock complicating acute myocardial infarction in the elderly: Predictors of long-term s...