Document Detail


Recognition of synthetic polypeptides corresponding to the N- and C-terminal fragments of Plasmodium falciparum Exp-1 by T-cells and plasma from human donors from African endemic areas.
MedLine Citation:
PMID:  11982859     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present work describes the recognition of three synthetic polypeptides encompassing the N- and C-terminal regions of the transmembrane Exp-1 protein of the parasite Plasmodium falciparum by plasma and peripheral blood mononuclear cells from naturally exposed individuals living in African endemic areas. The three polypeptides comprise the sequences 23-105, 73-162 and 101-162, and overlap at the transmembrane domain (73-105). Thus, they permitted characterization of the immune response specific to the N- and C-terminal domains in an independent fashion. Two different populations were evaluated, one in the village of Safo in Mali and the other in the villages of Somnaway, Kabortenga and Toussouktenga in Burkina Faso. Antibodies to the sequence 73-162 of Pf Exp-1 were found in 70% of adult Mali donors and in all of the donors tested from Burkina Faso. Strikingly, the N-terminal fragment Pf Exp-1 23-105 was only weakly recognized by a few donors. Evaluation of the T-cell response indicated that the peptide Pf Exp-1 23-105 was more potent than Pf Exp-1 73-162 in inducing a proliferative response. A correlation between peptide-specific interferon-gamma and interleukin-6 production and proliferation to peptide Pf Exp-1 23-105 was observed. Further studies are needed to evaluate this molecule as a vaccine candidate.
Authors:
Valentin Meraldi; Issa Nebié; Rémy Moret; Nadine Cuzin-Ouattara; Ali Thiocone; Ogobara Doumbo; Fulvio Esposito; Alfred S Traoré; Giampietro Corradin; Silvia Terenzi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Parasite immunology     Volume:  24     ISSN:  0141-9838     ISO Abbreviation:  Parasite Immunol.     Publication Date:  2002 Mar 
Date Detail:
Created Date:  2002-05-01     Completed Date:  2002-08-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7910948     Medline TA:  Parasite Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  141-50     Citation Subset:  IM    
Affiliation:
Institute of Biochemistry, University of Lausanne, Epalinges, Switzerland.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Africa / epidemiology
Animals
Antibodies, Protozoan / blood,  immunology*
Antigens, Protozoan / chemistry*,  immunology*
Burkina Faso / epidemiology
Cells, Cultured
Child, Preschool
Endemic Diseases
Female
Humans
Malaria, Falciparum / epidemiology,  immunology*
Male
Mali / epidemiology
Peptide Fragments / chemistry,  immunology
Plasmodium falciparum / immunology*
T-Lymphocytes / immunology*
Chemical
Reg. No./Substance:
0/Antibodies, Protozoan; 0/Antigens, Protozoan; 0/Peptide Fragments; 0/QF116 antigen, Plasmodium falciparum

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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