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Recognition of heat-shock protein 60 epitopes in children with type 1 diabetes.
MedLine Citation:
PMID:  22492505     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
BACKGROUND: Treatment with a specific HSP60 epitope in new onset type 1 diabetes (T1D) patients has been shown to preserve endogenous insulin production. Previously, recognition of pan HLA-DR binding HSP60 epitopes in various auto-immune diseases was found; this study investigated recognition of these epitopes in newly diagnosed T1D patients and correlated findings to the occurrence of a partial remission (PR). METHODS: PBMCs of 18 children with T1D were prospectively collected at disease onset and a few months after diagnosis. Epitope-specific T cell proliferation and cytokine production (intracellular and in culture supernatants) were measured; results were compared to 31 longstanding T1D patients and 10 healthy controls (HC). RESULTS: Although HSP60-epitope specific T cell proliferative responses were detected, overall proliferative responses were low. At onset, epitope-specific intracellular IFN-y production was higher in T1D patients compared to HC (p < 0.05). At follow-up, both IL-10 and IFN-γ production were higher in those without than in those with a PR (both p < 0.05). Also, IL-10 and IFN-γ production were higher compared to onset for patients without a PR (both p < 0.01). In supernatants of HSP60 epitope-specific T cell cultures, no substantial differences in cytokine production were found between T1D patients with and without a PR, either at onset or a few months after onset. As patient numbers were small, results should be interpreted with caution. CONCLUSIONS: PanDR binding HSP60 peptides induced low peptide-specific proliferative responses and peptide-specific production of some, mainly intracellular, cytokines in T1D patients. Recognition did not differ significantly between patient groups or various time points. Copyright © 2012 John Wiley & Sons, Ltd.
Authors:
A A Verrijn Stuart; W de Jager; M R Klein; G Teklenburg; R Nuboer; J J G Hoorweg; M A M J de Vroede; I de Kruijff; M Fick; E J Schroor; G J van der Vlist; J Meerding; S Kamphuis; B J Prakken
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-10
Journal Detail:
Title:  Diabetes/metabolism research and reviews     Volume:  -     ISSN:  1520-7560     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100883450     Medline TA:  Diabetes Metab Res Rev     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 John Wiley & Sons, Ltd.
Affiliation:
Department of Paediatric Endocrinology, University Medical Center Utrecht, the Netherlands; Department of Paediatric Immunology and Centre for Cellular and Molecular Intervention, University Medical Center Utrecht, the Netherlands. a.a.verrijnstuart@umcutrecht.nl.
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