| Recognition of acidic phospholipase A2 activity in plasma membranes of resident peritoneal macrophages. | |
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MedLine Citation:
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PMID: 3137407 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Phospholipase (PLase) activities in the plasma membrane of guinea pig peritoneal macrophages were studied, as these enzymes having such activity may be candidates for the release of arachidonic acid (AA) from phosphatidylcholine (PC). An AA release system operating at acidic pH was identified in the macrophage plasma membrane and characterized. This membrane-bound acidic PLase A2 had an optimum pH at 4.5, and enzyme activation was observed in Ca++-free medium; but the maximum activity was found at 0.5 mM Ca++ concentration. The Km value for PC of acidic PLase A2 was 4.2 microM, and a Michaelis-Menten relationship was evident. Calcium might act as a cofactor at some intermediate step during the activation of acidic PLase A2 in light of the uncompetitive manner of Ca++ action. Furthermore, the release of [3H]-AA from preradiolabelled macrophage plasma membranes occurred with the addition of Ca++ at pH 4.5. These data suggest that the acid PLase A2 is a component of the plasma membrane and is not due to lysosomal contamination since membrane-bound acidic PLase A2 properties are opposite to those found for lysosomal PLase A2. |
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Authors:
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Y Shibata; Y Abiko; H Ohno; T Araki; H Takiguchi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Life sciences Volume: 43 ISSN: 0024-3205 ISO Abbreviation: Life Sci. Publication Date: 1988 |
Date Detail:
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Created Date: 1988-10-07 Completed Date: 1988-10-07 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0375521 Medline TA: Life Sci Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 889-96 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Nihon University School of Dentistry, Matsudo, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arachidonic Acid Arachidonic Acids / metabolism Calcium / pharmacology Cell Membrane / enzymology Fatty Acids / metabolism Guinea Pigs Hydrogen-Ion Concentration Kinetics Macrophages / enzymology* Male Peritoneal Cavity / cytology Phospholipases / metabolism* Phospholipases A / metabolism* Phospholipases A2 Substrate Specificity |
| Chemical | |
Reg. No./Substance:
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0/Arachidonic Acids; 0/Fatty Acids; 506-32-1/Arachidonic Acid; 7440-70-2/Calcium; EC 3.1.-/Phospholipases; EC 3.1.1.-/Phospholipases A; EC 3.1.1.4/Phospholipases A2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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