Document Detail

Recognition of Naegleriae ameba surface protein epitopes by anti-human CD45 antibodies.
MedLine Citation:
PMID:  20151453     Owner:  NLM     Status:  MEDLINE    
Phagocytosis is a highly conserved mechanism exhibited by both free-living amebas and mammalian blood cells. Similarities demonstrated by either cell type during engulfment of the same bacterial species may imply analogous surface proteins involved in receptor-mediated endocytosis. The increased availability of anti-human leukocyte antibodies or clusters of differentiation (CD) markers used in conjunction with flow cytometric (FCM) and/or immunohistochemical (IHC) analysis provides investigators with a relatively easy method to screen different cell populations for comparable plasma membrane proteins. In this study, we incubated Naegleria and Acanthamoeba amebas with several directly conjugated anti-human leukocyte monoclonal antibodies (mAb) for similarly recognized amebic epitopes. CD marker selection was based upon a recognized role of each mAb in phagocyte activation and/or uptake of bacteria. These included CD14, CD45, and CD206. In FCM, only one CD45 antibody demonstrated strong reactivity with both Naegleria fowleri and Naegleria gruberi that was not expressed in similarly tested Acanthamoeba species. Additional testing of N. gruberi by IHC demonstrated reactivity to a different CD45 antibody. Our results suggest a possible utility of using anti-human leukocyte antibodies to screen amebic cells for similarly expressed protein epitopes. In doing so, several important items must be considered when selecting potential mAbs for testing to increase the probability of a positive result.
Terrence J Ravine; Jacek M Polski; James Jenkins
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cytometry. Part A : the journal of the International Society for Analytical Cytology     Volume:  77     ISSN:  1552-4930     ISO Abbreviation:  Cytometry A     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-25     Completed Date:  2010-06-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101235694     Medline TA:  Cytometry A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  305-9     Citation Subset:  IM    
Department of Biomedical Sciences, University of South Alabama, Mobile, Alabama 36688, USA.
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MeSH Terms
Antibodies, Monoclonal / immunology*
Antigens, CD45 / immunology*
Cell Membrane / metabolism
Epitopes / immunology*
Flow Cytometry
Membrane Proteins / immunology*
Naegleria / cytology*,  immunology*
Protozoan Proteins / immunology*
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Epitopes; 0/Membrane Proteins; 0/Protozoan Proteins; EC, CD45

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