| Recognition of intermediate functionality by acyl carrier protein over a complete cycle of fatty acid biosynthesis. | |
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MedLine Citation:
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PMID: 20659690 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It remains unclear whether in a bacterial fatty acid synthase (FAS) acyl chain transfer is a programmed or diffusion controlled and random action. Acyl carrier protein (ACP), which delivers all intermediates and interacts with all synthase enzymes, is the key player in this process. High-resolution structures of intermediates covalently bound to an ACP representing each step in fatty acid biosynthesis have been solved by solution NMR. These include hexanoyl-, 3-oxooctanyl-, 3R-hydroxyoctanoyl-, 2-octenoyl-, and octanoyl-ACP from Streptomyces coelicolor FAS. The high-resolution structures reveal that the ACP adopts a unique conformation for each intermediate driven by changes in the internal fatty acid binding pocket. The binding of each intermediate shows conserved structural features that may ensure effective molecular recognition over subsequent rounds of fatty acid biosynthesis. |
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Authors:
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Eliza Płoskoń; Christopher J Arthur; Amelia L P Kanari; Pakorn Wattana-amorn; Christopher Williams; John Crosby; Thomas J Simpson; Christine L Willis; Matthew P Crump |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Chemistry & biology Volume: 17 ISSN: 1879-1301 ISO Abbreviation: Chem. Biol. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-07-27 Completed Date: 2010-11-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9500160 Medline TA: Chem Biol Country: United States |
Other Details:
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Languages: eng Pagination: 776-85 Citation Subset: IM |
Copyright Information:
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2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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School of Chemistry, University of Bristol, Cantock's Close, Bristol BS81TS, United Kingdom. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acyl Carrier Protein
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chemistry,
metabolism* Amino Acid Sequence Bacterial Proteins / chemistry, metabolism* Fatty Acids / biosynthesis* Hydrophobicity Models, Molecular Molecular Sequence Data Pantetheine / analogs & derivatives, metabolism Protein Conformation Streptomyces coelicolor / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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BB/F014570/1//Biotechnology and Biological Sciences Research Council; WT082352MA//Wellcome Trust |
| Chemical | |
Reg. No./Substance:
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0/Acyl Carrier Protein; 0/Bacterial Proteins; 0/Fatty Acids; 2226-71-3/4'-phosphopantetheine; 496-65-1/Pantetheine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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