Document Detail

Reciprocal regulation of alpha-fetoprotein and albumin gene expression by butyrate in human hepatoma cells.
MedLine Citation:
PMID:  7518784     Owner:  NLM     Status:  MEDLINE    
BACKGROUND/AIMS: Butyrate, a product of colonic bacterial flora, functions as an antiproliferative agent and induces cell differentiation in a variety of cell types. In the present study, the effects of butyrate on cell growth and expression of alpha-fetoprotein (AFP) and albumin genes in HuH-7 human hepatoma cells were investigated. METHODS: The HuH-7 cells were treated with sodium butyrate (0-1 mmol/L), and numbers of viable cells were counted at 24, 48, and 72 hours after treatment. To elucidate the effects of sodium butyrate on AFP and albumin gene expression, Northern blotting and transient chloramphenicol acetyltransferase plasmid transfection experiments were performed. RESULTS: Cell growth was dose dependently inhibited by sodium butyrate. By Northern blot analysis, the level of AFP messenger RNA was reduced by treatment with sodium butyrate, whereas the level of albumin messenger RNA was elevated by this treatment. In transient chloramphenicol acetyltransferase plasmid transfection experiments, sodium butyrate repressed the AFP promoter activity but did not change the AFP enhancer or silencer activities. In contrast, the albumin promoter activity was stimulated by sodium butyrate. CONCLUSIONS: These results suggest that butyrate leads to the reciprocal differentiating regulation of AFP and albumin gene expression at the transcriptional level in human hepatoma cells.
T Tsutsumi; A Ido; K Nakao; K Hamasaki; Y Kato; A Ohtsuru; K Nakata; T Tamaoki; S Nagataki
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Gastroenterology     Volume:  107     ISSN:  0016-5085     ISO Abbreviation:  Gastroenterology     Publication Date:  1994 Aug 
Date Detail:
Created Date:  1994-08-25     Completed Date:  1994-08-25     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0374630     Medline TA:  Gastroenterology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  499-504     Citation Subset:  AIM; IM    
First Department of Internal Medicine, Nagasaki University School of Medicine, Japan.
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MeSH Terms
Albumins / genetics*
Blotting, Northern
Butyric Acid
Butyric Acids / pharmacology*
Carcinoma, Hepatocellular / genetics*,  metabolism,  pathology
Cell Division / drug effects
Chloramphenicol O-Acetyltransferase / genetics
Gene Expression Regulation, Neoplastic / drug effects*
Liver Neoplasms / genetics*,  metabolism,  pathology
Plasmids / genetics
Promoter Regions, Genetic / drug effects
RNA, Messenger / metabolism
Transcription, Genetic / drug effects
Tumor Cells, Cultured / drug effects,  metabolism,  pathology
alpha-Fetoproteins / genetics*
Reg. No./Substance:
0/Albumins; 0/Butyric Acids; 0/RNA, Messenger; 0/alpha-Fetoproteins; 107-92-6/Butyric Acid; EC O-Acetyltransferase

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