Document Detail

Receptor-mediated gene delivery by folate-poly(ethylene glycol)-grafted-trimethyl chitosan in vitro.
MedLine Citation:
PMID:  20964597     Owner:  NLM     Status:  In-Data-Review    
Folate-poly(ethylene glycol)-grafted-trimethyl chitosan (F-PEG-g-TMC) and methoxypolyethylene glycol-grafted-trimethyl chitosan (mPEG-g-TMC)/pDNA complexes were prepared and characterized concerning physicochemical properties including cytotoxicity, condensation efficiency, particle size, and zeta potential. Furthermore, cellular uptake and transfection efficiency of the complexes were evaluated in vitro and compared with that of folate-trimethyl chitosan (folate-TMC) synthesized by our group to elucidate the effect of PEGylation. The cellular uptake of the F-PEG-g-TMC/pDNA with a copolymer nitrogen-to-DNA phosphate ratio (N/P ratio) of 20 in KB cells was specifically increased up to 1.68-fold compared with that of the mPEG-g-TMC/pDNA (N/P ratio 20) resulting in 1.5-fold and 1.4-fold increased transfection efficiency in KB cells and SKOV3 cells (folate receptor-overexpressing cell lines), respectively. The intracellular uptake and transfection efficiency of the F-PEG-g-TMC/pDNA were significantly enhanced relative to the folate-TMC/pDNA in folate receptor-overexpressing cells due to stabilizing effect of PEGylation. Subcellular localization of the complexes in the process of intracellular transportation was observed by confocal laser scanning microscopy suggesting quicker association of the F-PEG-g-TMC/pDNA. In conclusion, the F-PEG-g-TMC/pDNA complexes are potential vehicles for improving the transfection efficiency and specificity of gene.
Yu Zheng; Xiangrong Song; Gu He; Zheng Cai; Yan Zhou; Bo Yu; Jiapeng Xu; Yuquan Wei; Shixiang Hou
Related Documents :
19759827 - High efficiency lipid-based sirna transfection of adipocytes in suspension.
15587917 - Stable transfection of mdck cells with epitope-tagged human pept1.
12054557 - A two step model aimed at delivering antisense oligonucleotides in targeted cells.
2767047 - Joining of linear plasmid dna is reduced and error-prone in bloom's syndrome cells.
2032987 - Nonxenobiotic manipulation and sulfur precursor specificity of human endothelial cell g...
23775237 - On the early development of organic dyes for dye-sensitized solar cells.
Publication Detail:
Type:  Journal Article     Date:  2010-10-22
Journal Detail:
Title:  Journal of drug targeting     Volume:  19     ISSN:  1029-2330     ISO Abbreviation:  J Drug Target     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-08-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9312476     Medline TA:  J Drug Target     Country:  England    
Other Details:
Languages:  eng     Pagination:  647-56     Citation Subset:  IM    
State Key Laboratory of Biotherapy, West China Hospital.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Reconstructive endovascular treatment of intracranial aneurysms with the Willis covered stent: mediu...
Next Document:  Regulation of Human Mesenchymal Stem Cell Functions by an Autocrine Loop Involving NAD(+) Release an...