Document Detail


Receptor-mediated clearance of tissue kallikreins by rat liver.
MedLine Citation:
PMID:  3643709     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The exsanguinated, isolated and perfused rat liver clears from the perfusate, at comparable rates, some native tissue kallikreins: human and horse urinary as well as hog pancreatic; the clearance rates were dependent on the initial enzyme concentration in the perfusing fluid. Contrary-wise, rat urinary kallikrein was cleared at negligible rates. Neuraminidase pretreatment of these four kallikreins did not alter their clearance rates. Horse urinary kallikrein binding to isolated prefixed hepatocytes was calcium-dependent and inhibited by asialofetuin (but not by fetuin) and some sugars; these characteristics are compatible with the interpretation that this native tissue kallikrein is recognized by the hepatocyte asialoglycoprotein-receptor. It was calculated that there are about 300,000 receptor sites per cell either using perfusion experiments at 4 degrees C or isolated hepatocytes.
Authors:
D R Borges; M Kouyoumdjian; E S Prado; J L Prado
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Advances in experimental medicine and biology     Volume:  198 Pt A     ISSN:  0065-2598     ISO Abbreviation:  Adv. Exp. Med. Biol.     Publication Date:  1986  
Date Detail:
Created Date:  1987-03-11     Completed Date:  1987-03-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0121103     Medline TA:  Adv Exp Med Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  235-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Asialoglycoprotein Receptor
Horses
Kallikreins / metabolism*,  urine
Kinetics
Liver / metabolism*
Perfusion
Rats
Receptors, Immunologic / metabolism*
Chemical
Reg. No./Substance:
0/Asialoglycoprotein Receptor; 0/Receptors, Immunologic; EC 3.4.21.-/Kallikreins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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