| Receptor-binding sites on C3 and C3b. | |
| | |
MedLine Citation:
|
PMID: 864256 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Human erythrocytes carry C3b receptors: Daudi lymphoid cells carry exclusively C3d receptors. With these two types of cells, it could be shown that isolated, uncleaved human C3 and soluble C3b possess two stable binding sites: SBS1-specific C3b receptors, and SBS2-specific for C3d receptors. Upon binding of freshly cleaved C3b (nascent C3b) via its labile binding site (generated on C3b through cleavage of C3) to the C3 acceptors on cell surfaces, the SBS2 becomes concealed. Kinetic experiments show that immediately after the action of fetal calf serum or partially purified C3b-inactivator on EAC1423b the SBS2 is accessible again. The reappearance of SBS2 does coincide with cleavage of surface-bound C3b into C3c and C3d but not with the release of C3c from the cell; C3c remains attached and is released only with delay. Concomitant with the cleavage event the number of SBS1 is reduced, stressing the importance of an unaltered steric configuration of the C3b structure for the expression of SBS1. An alternative explanation might be that the SBS1 is located at the site connecting C3c and C3d, so that it becomes altered upon dissection of the two fragments. |
| | |
Authors:
|
M P Dierich; V A Bokisch |
Related Documents
:
|
11414366 - Ficolins and the lectin complement pathway. 18313756 - Highly specific inhibition of c1q globular-head binding to human igg: a novel approach ... 12085326 - Onchocerca volvulus microfilariae avoid complement attack by direct binding of factor h. 1448966 - Complement activation by cold agglutinins. 9016316 - Recombinant galectin-1 recognizes mucin and epithelial cell surface glycocalyces of gas... 11292376 - Molecular interactions of syndecans during development. |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Journal of immunology (Baltimore, Md. : 1950) Volume: 118 ISSN: 0022-1767 ISO Abbreviation: J. Immunol. Publication Date: 1977 Jun |
Date Detail:
|
Created Date: 1977-07-29 Completed Date: 1977-07-29 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 2985117R Medline TA: J Immunol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 2145-50 Citation Subset: AIM; IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Binding Sites, Antibody Cell Membrane / immunology Complement C3 / analysis, biosynthesis, metabolism* Complement System Proteins / metabolism* Humans Immune Adherence Reaction Solubility |
| Chemical | |
Reg. No./Substance:
|
0/Binding Sites, Antibody; 0/Complement C3; 9007-36-7/Complement System Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Suppressor lymphocytes in young and aged humans.
Next Document: Kinetic studies on the fragmentation of the third component of complement (C3) by trypsin.