Document Detail

The Receptor for Advanced Glycation End-products (RAGE) protects pancreatic tumor cells against oxidative injury.
MedLine Citation:
PMID:  21126167     Owner:  NLM     Status:  MEDLINE    
Reactive oxygen species, including hydrogen peroxide (H(2)O(2)), can cause toxicity and act as signaling molecules in various pathways regulating both cell survival and cell death. However, the sequence of events between the oxidative insult and cell damage remains unclear. In the current study, we investigated the effect of oxidative stress on activation of the Receptor for Advanced Glycation End-products (RAGE) and subsequent protection against H(2)O(2)-induced pancreatic tumor cell damage. We found that exposure of pancreatic tumor cells to H(2)O(2) provoked a nuclear factor kappa B (NF-κB)-dependent increase in RAGE expression. Further, suppression of RAGE expression by RNA interference increased the sensitivity of pancreatic tumor cells to oxidative injury. Furthermore, targeted knockdown of RAGE led to increased cell death by apoptosis and diminished cell survival by autophagy during H(2)O(2)-induced oxidative injury. Moreover, we demonstrate that RAGE is a positive feedback regulator for NF-κB as knockdown of RAGE decreased H(2)O(2)-induced activity of NF-κB. Taken together, these results suggest that RAGE is an important regulator of oxidative injury.
Rui Kang; Daolin Tang; Kristen M Livesey; Nicole E Schapiro; Michael T Lotze; Herbert J Zeh
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-04-21
Journal Detail:
Title:  Antioxidants & redox signaling     Volume:  15     ISSN:  1557-7716     ISO Abbreviation:  Antioxid. Redox Signal.     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-09-02     Completed Date:  2012-03-21     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  100888899     Medline TA:  Antioxid Redox Signal     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2175-84     Citation Subset:  IM    
Department of Surgery, Hillman Cancer Center, University of Pittsburgh Cancer Institute, 5117 Centre Avenue, Pittsburgh, PA 15213, USA.
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MeSH Terms
Apoptosis / drug effects,  genetics
Autophagy / drug effects,  genetics
Blotting, Western
Cell Line, Tumor
Cell Survival / drug effects,  genetics
Hydrogen Peroxide / pharmacology
NF-kappa B / metabolism
Oxidative Stress / genetics,  physiology
Pancreatic Neoplasms / metabolism*
RNA Interference
Real-Time Polymerase Chain Reaction
Receptors, Immunologic / genetics,  metabolism*
Signal Transduction / genetics,  physiology
Grant Support
1 P01 CA 101944-04/CA/NCI NIH HHS
Reg. No./Substance:
0/NF-kappa B; 0/Receptors, Immunologic; 0/advanced glycosylation end-product receptor; 7722-84-1/Hydrogen Peroxide

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