| Recent advances in the MS analysis of glycoproteins: Capillary and microfluidic workflows. | |
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MedLine Citation:
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PMID: 21110330 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Recent developments in bioanalytical instrumentation, MS detection, and computational data analysis approaches have provided researchers with capabilities for interrogating the complex cellular glycoproteome, to help gain a better insight into the cellular and physiological processes that are associated with a disease and to facilitate the efforts centered on identifying disease-specific biomarkers. This review describes the progress achieved in the characterization of protein glycosylation by using advanced capillary and microfluidic MS technologies. The major steps involved in large-scale glycoproteomic analysis approaches are discussed, with special emphasis given to workflows that have evolved around complex MS detection functions. In addition, quantitative analysis strategies are assessed, and the bioinformatics aspects of glycoproteomic data processing are summarized. The developments in commercial and custom fabricated microfluidic front-end platforms to ESI- and MALDI-MS instrumentation, for addressing major challenges in carbohydrate analysis such as sensitivity, throughput, and ability to perform structural characterization, are further evaluated and illustrated with relevant examples. |
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Authors:
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Diego F Cortes; Jarod L Kabulski; Alexandru C Lazar; Iulia M Lazar |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2010-11-25 |
Journal Detail:
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Title: Electrophoresis Volume: - ISSN: 1522-2683 ISO Abbreviation: - Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-11-26 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8204476 Medline TA: Electrophoresis Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Virginia Bioinformatics Institute and Virginia Polytechnic Institute and State University, Blacksburg, VA, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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