| Recellularization of aortic valves in pigs. | |
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MedLine Citation:
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PMID: 21055964 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Objectives: Decellularized porcine heart valves treated with deoxycholic acid (DOA) have demonstrated complete recellularization and absence of calcification when implanted into the pulmonary position in sheep. We studied recellularization and calcification in stented DOA-treated heart valves compared with conventional stented glutaraldehyde-treated valves in the aortic position in juvenile pigs 6 months after implantation. Methods: DOA heart valves (n=12) and glutaraldehyde-treated valves (Carpentier-Edwards) (n=15) were implanted into the aortic position in 8-month old 90kg female pigs. Six months postoperatively, the valves were explanted and subjected to gross pathology examination, high-resolution (HR) X-ray imaging, and histological evaluation. Results: Five DOA valves and five glutaraldehyde-treated valves were explanted after 6 months. Fourteen animals died before follow-up because of non-valve related causes and three because of infective endocarditis. Gross pathologic examination showed all DOA valves to be well functioning with only minor thrombotic depositions located mostly in the commissural area. Three glutaraldehyde valves had limited thrombosis and two had severe thrombosis. HR X-ray imaging demonstrated almost complete absence of cusp calcification in the DOA valves, but severe calcification in all glutaraldehyde valves. Overgrowth of endothelial cells and ingrowth of fibroblasts in the stent-adjacent area and basal part of the cusps were seen in all DOA valves, but not in glutaraldehyde valves. Immunohistochemistry revealed larger amounts of inflammatory cells in all glutaraldehyde valves compared with DOA valves. Conclusions: DOA-treated heart valves demonstrated greater recellularization and less calcification compared with standard glutaraldehyde-treated valves 6 months after implantation in the aortic position in pigs. DOA-treated heart valves demonstrated less calcification compared with standard glutaraldehyde-treated valves by qualitative analysis. Endothelial and fibroblast recellularization of the cusps was only observed in DOA-treated valves. |
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Authors:
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Jesper Langhoff Honge; Jonas Funder; Erik Hansen; Pascal Maria Dohmen; Wolfgang Konertz; John Michael Hasenkam |
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Publication Detail:
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Type: Journal Article Date: 2010-11-04 |
Journal Detail:
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Title: European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery Volume: 39 ISSN: 1873-734X ISO Abbreviation: Eur J Cardiothorac Surg Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-05-09 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8804069 Medline TA: Eur J Cardiothorac Surg Country: Germany |
Other Details:
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Languages: eng Pagination: 829-34 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 European Association for Cardio-Thoracic Surgery. Published by Elsevier B.V. All rights reserved. |
Affiliation:
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Department of Cardiothoracic and Vascular Surgery, Aarhus University Hospital, Skejby, Denmark; Clinical Institute, Aarhus University Hospital, Skejby, Denmark. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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