Document Detail


Recalibrating the gram stain diagnosis of male urethritis in the era of nucleic Acid amplification testing.
MedLine Citation:
PMID:  22183839     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
BACKGROUND: : Criteria for diagnosis of male urethritis based on a Gram-stained smear (GSS) of urethral discharge were developed before the era of nucleic acid amplification testing (NAAT) for Neisseria gonorrhoeae (Ng) and Chlamydia trachomatis (Ct) and may be too stringent when considering the higher sensitivity of NAAT.
OBJECTIVE: : To evaluate Ct and Ng positivity at different strata of polymorphonuclear cells (PMN) per high power field (hpf) GSS microscopy.
METHODS: : A retrospective analysis of the electronic medical record system of the Denver Metro Health Clinic between March 1, 2005 and December 31, 2010; all men with a GSS test during this period had an NAAT for Ct and Ng and had GSS results recorded at 1 PMN/hpf increments.
RESULTS: : A total of 13,520 GSS were available for analysis. For Ct, a statistically significant trend was observed along the PMN/hpf incremental spectrum, and a significant increase in positivity was observed between the 1 and 2 PMN/hpf strata (from 6.5% to 16.2%). For men diagnosed with Ng, no such trend was observed, and >95% of GSS results fell in the >10 strata. A subanalysis to control for laboratory technician variance and difference in NAAT technology showed similar results.
CONCLUSION: : Our data support lowering the diagnostic criteria of the GSS diagnosis of male urethritis to ≥2 PMN/hpf. At this level, the Ct positivity (16.2%) is similar or higher than positivity in men who receive presumptive chlamydia treatment as a contact to patients diagnosed with gonorrhea, pelvic inflammatory disease, or mucopurulent cervicitis.
Authors:
Cornelis A Rietmeijer; Christie J Mettenbrink
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Sexually transmitted diseases     Volume:  39     ISSN:  1537-4521     ISO Abbreviation:  Sex Transm Dis     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2011-12-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7705941     Medline TA:  Sex Transm Dis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  18-20     Citation Subset:  IM    
Affiliation:
From the *Denver Public Health Department and †Colorado School of Public Health, University of Colorado Denver, Denver, Colorado.
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