Document Detail


Rearrangements of ribosomal DNA clusters in late generation telomerase-deficient Arabidopsis.
MedLine Citation:
PMID:  14579127     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The ends of eukaryotic chromosomes are capped with special nucleoprotein structures called telomeres. Telomere shortening due to telomerase inactivation may result in fusion of homologous or heterologous chromosomes, leading to their successive breakage during anaphase movement, followed by fusion of broken ends in the next cell cycle, i.e. the breakage-fusion-bridge (BFB) cycle. Using fluorescence in situ hybridization (FISH) with 25S rDNA and specific bacterial artificial chromosome (BAC) probes we demonstrate participation of chromosomes 2 and 4 of Arabidopsis thaliana AtTERT null plants in the formation of anaphase bridges. Both homologous and non-homologous chromosomes formed transient anaphase bridges whose breakage and unequal separation led to genome rearrangement, including non-reciprocal translocations and aneuploidy. The 45S rDNA regions located at the ends of chromosomes 2 and 4 were observed in chromosome bridges at a frequency approximately ten times higher than expected in the case of random fusion events. This outcome could result from a functional association of rDNA repeats at nucleoli. We also describe increased variation in the number of nucleoli in some interphase cells with supernumerary rDNA FISH signals. These data indicate that dysfunctional telomeres in Arabidopsis lead to massive genome instability, which is induced by multiple rounds of the BFB mechanism.
Authors:
Jiri Siroky; Jitka Zluvova; Karel Riha; Dorothy E Shippen; Boris Vyskot
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2003-09-17
Journal Detail:
Title:  Chromosoma     Volume:  112     ISSN:  0009-5915     ISO Abbreviation:  Chromosoma     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2003-10-27     Completed Date:  2004-07-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985138R     Medline TA:  Chromosoma     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  116-23     Citation Subset:  IM    
Affiliation:
Laboratory of Plant Developmental Genetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska 135, 612 65 Brno, Czech Republic.
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MeSH Terms
Descriptor/Qualifier:
Anaphase / genetics
Arabidopsis / genetics*
Cell Nucleolus / metabolism
Chromosomes, Artificial, Bacterial
Chromosomes, Plant / genetics,  metabolism*
DNA, Ribosomal / genetics
Gene Rearrangement / genetics*
Genomic Instability*
In Situ Hybridization, Fluorescence
Telomerase / deficiency*
Telomere / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/DNA, Ribosomal; EC 2.7.7.49/Telomerase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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