Document Detail


Rearrangement of esophageal-carcinoma cells and stromal fibroblasts in a multicellular spheroid.
MedLine Citation:
PMID:  21552906     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Using a thermo-responsive polymer, a collagen-conjugated poly-N-isopropyl acrylamide (PNIPAAm) as a substratum, we developed hetero-multicellular spheroids (MCS) composed of esophageal squamous carcinoma cells (TE10) and esophageal fibroblasts isolated from esophageal carcinoma tissue. PNIPAAm is insoluble in water above the lower critical solution temperature (LCST; about 32 degrees C) and becomes reversibly solubilized below the LCST. Taking advantage of this conversion, we prepared three types of hetero-MCS as follows: F/T-multicellular spheroids made by seeding of TE10 onto a preprepared monolayer of fibroblasts; T/F-multicellular spheroids made by seeding of fibroblasts onto a preprepared monolayer of TE10; Mixed-multicellular spheroid made from a monolayer of mixed fibroblasts and TE10. Histolo,oical and immunohistochemical examinations revealed that fibroblasts and TE10 cells were intermingled in 5-day-old multicellular spheroids but were divided into three zones in 1- or 2 week-cultured spheroids: into an external zone composed almost entirely of TE10 cells that were positive for epithelial membrane antigen (EMA), an intermediate zone composed of fibroblasts that were positive for vimentin, and a necrotic zone showing variable evidence of cell injury. This distribution was observed in all three types of the spheroids described above. These findings indicate that TE10 cells are able to migrate and cover the surface to make organizing spheroid, thus mimicking in vivo structures. We conclude that the three-dimensional culture system using a thermo-responsive polymer, which enables coculturing with different types of cells as a heteromilticellular spheroid, is a useful model to examine the interaction between carcinoma cells and their stroma cells as it occurs in vivo.
Authors:
I Shima; S Kubota; Y Sasaguri; T Hamada; N Arima; A Ito; M Morimatsu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of oncology     Volume:  7     ISSN:  1019-6439     ISO Abbreviation:  Int. J. Oncol.     Publication Date:  1995 Oct 
Date Detail:
Created Date:  2011-05-09     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9306042     Medline TA:  Int J Oncol     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  795-800     Citation Subset:  -    
Affiliation:
UNIV OCCUPAT & ENVIRONM HLTH,SCH MED,DEPT PATHOL,YAHATANISHI KU,KITAKYUSHU,FUKUOKA 807,JAPAN. KURUME UNIV,SCH MED,DEPT PATHOL,KURUME,FUKUOKA 830,JAPAN. ST MARIANNA UNIV,SCH MED,DEPT SURG,KAWASAKI,KANAGAWA 216,JAPAN. TOKYO COLL PHARM,DEPT BIOCHEM,HACHIOJI 19203,TOKYO,JAPAN.
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