Document Detail

Reanalysis of the protocol for in vitro synchronization of mammalian astrocytic cultures by serum deprivation.
MedLine Citation:
PMID:  15979934     Owner:  NLM     Status:  MEDLINE    
Serum starvation of astrocytes for a period of time followed by refeeding has been proposed as a method to produce synchronized astrocytes. Here, it is proposed that the method neither synchronizes cells nor satisfies rigorous criteria for cell synchronization. The proposed non-selective, whole-culture synchronization protocol cannot, in theory, synchronize cells. The cells produced by the proposed serum starvation/refeeding protocol do not reflect the properties of any particular cell during the cell cycle. Cells produced by the published protocol will have a wide distribution of cell sizes, and therefore, the cells produced by starvation/refeeding will not model cells of any specific age during the division cycle. Thus, the proposed protocol will not produce a synchronized culture.
Stephen Cooper
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Brain research. Brain research protocols     Volume:  15     ISSN:  1385-299X     ISO Abbreviation:  Brain Res. Brain Res. Protoc.     Publication Date:  2005 Sep 
Date Detail:
Created Date:  2005-09-16     Completed Date:  2005-11-15     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9716650     Medline TA:  Brain Res Brain Res Protoc     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  115-8     Citation Subset:  IM    
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109-0620, USA.
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MeSH Terms
Astrocytes / cytology*
Cell Culture Techniques / methods
Cell Cycle*
Culture Media, Serum-Free
Grant Support
5 P30 CA46592/CA/NCI NIH HHS
Reg. No./Substance:
0/Culture Media, Serum-Free

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