Document Detail


Realgar-mediated growth inhibition on HaCaT human keratinocytes is associated with induction of apoptosis.
MedLine Citation:
PMID:  19578792     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Traditional Chinese medicine has long been used to treat a variety of ailments including skin diseases. Our previous study has revealed the ethanolic extract of realgar, a common ingredient used in psoriasis treatment in Chinese medicine, to possess potent anti-proliferative action on cultured HaCaT cells of human keratinocyte origin. In the present study, the mechanisms of action of the observed growth inhibitory action of realgar were investigated. Several bioassay methods were employed to elucidate whether cellular apoptosis is involved in the realgar-induced growth inhibition of the skin cells. Morphologically, nuclear condensation and DNA fragmentation were observed when HaCaT cells were exposed to the realgar extract. DNA fragmentation induced by the treatment of realgar was also evident as detected by gel electrophoresis and the TUNEL method. Cell cycle analysis by propidium iodide (PI) staining demonstrated the appearance of sub-G1 peak and cell cycle arrest at the G1 phase upon realgar treatment. Quantitative analysis by annexin V-PI staining revealed that the realgar-induced apoptotic event was dose-dependent. Furthermore, realgar was able to activate caspase-3 expression when examined by Western blot analysis. Our experimental data unambiguously confirm that induction of cellular apoptosis is mainly responsible for the observed growth inhibition brought about by realgar on the HaCaT keratinocytes, and this finding helps place the traditional use of this mineral for psoriasis treatment on a scientific footing.
Authors:
Wai-Pui Tse; Christopher H K Cheng; Chun-Tao Che; Ming Zhao; Rui-Qiang Fan; Zhi-Xiu Lin
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of molecular medicine     Volume:  24     ISSN:  1107-3756     ISO Abbreviation:  Int. J. Mol. Med.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-07-06     Completed Date:  2009-09-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9810955     Medline TA:  Int J Mol Med     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  189-96     Citation Subset:  IM    
Affiliation:
School of Chinese Medicine, Faculty of Science, Department of Biochemistry, The Chinese University of Hong Kong, Shatin, Hong Kong, PR China.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / drug effects*
Arsenicals / pharmacology*
Blotting, Western
Caspase 3 / metabolism
Cell Line
Cell Proliferation / drug effects*
Cell Survival / drug effects
Chromatin / drug effects,  metabolism
DNA Fragmentation / drug effects
Dose-Response Relationship, Drug
Flow Cytometry
G1 Phase / drug effects
Humans
In Situ Nick-End Labeling
Keratinocytes / cytology,  drug effects*,  metabolism
Sulfides / pharmacology*
Time Factors
Chemical
Reg. No./Substance:
0/Arsenicals; 0/Chromatin; 0/Sulfides; 56320-22-0/arsenic disulfide; EC 3.4.22.-/Caspase 3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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