Document Detail

Real-time quantitative PCR in parasitology.
MedLine Citation:
PMID:  12377276     Owner:  NLM     Status:  Publisher    
Standard techniques for counting parasites are often time-consuming, difficult and inaccurate, and occasionally unpleasant. Real-time quantitative polymerase chain reaction has recently been applied to parasitology, specifically Plasmodium, Toxoplasma, Leishmania and Neospora. These techniques are truly quantitative, give results over a range of 6-7 orders of magnitude, are quick to perform and require no manipulations post-amplification. They can be used to count genome numbers and to study levels of gene expression. The advantages and limitations of existing thermocyclers and applicable detection systems are discussed here, and promising new developments are highlighted.
Andrew Bell; Lisa Ranford-Cartwright
Related Documents :
17447946 - Modeling of hormone secretion-generating mechanisms with splines: a pseudo-likelihood a...
8817906 - Accurate recovery of articulator positions from acoustics: new conclusions based on hum...
8746936 - Automated contour detection and acoustic quantification.
19063316 - Experiments to develop soundscape design methods.
18532496 - Speaker identification on the scotus corpus.
9543296 - Experimental models for studying mucociliary clearance.
Publication Detail:
Journal Detail:
Title:  Trends in parasitology     Volume:  18     ISSN:  1471-5007     ISO Abbreviation:  Trends Parasitol.     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-Oct-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100966034     Medline TA:  Trends Parasitol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  338     Citation Subset:  -    
Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow, G12 8QQ, Glasgow, UK
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Interaction of Leishmania with the host macrophage.
Next Document:  Extensive polymorphism and ancient origin of Plasmodium falciparum.