Document Detail


Real-time quantitative PCR in parasitology.
MedLine Citation:
PMID:  12377276     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Standard techniques for counting parasites are often time-consuming, difficult and inaccurate, and occasionally unpleasant. Real-time quantitative polymerase chain reaction has recently been applied to parasitology, specifically Plasmodium, Toxoplasma, Leishmania and Neospora. These techniques are truly quantitative, give results over a range of 6-7 orders of magnitude, are quick to perform and require no manipulations post-amplification. They can be used to count genome numbers and to study levels of gene expression. The advantages and limitations of existing thermocyclers and applicable detection systems are discussed here, and promising new developments are highlighted.
Authors:
Andrew Bell; Lisa Ranford-Cartwright
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Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Trends in parasitology     Volume:  18     ISSN:  1471-5007     ISO Abbreviation:  Trends Parasitol.     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-Oct-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100966034     Medline TA:  Trends Parasitol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  338     Citation Subset:  -    
Affiliation:
Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow, G12 8QQ, Glasgow, UK
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