| Real time polymerase chain reaction for the detection of malarial parasite. | |
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MedLine Citation:
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PMID: 22313646 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Objective: To evaluate the sensitivity and specificity of real time polymerase chain reaction (PCR) for the detection of Malarial parasite. Study Design: Descriptive cross-sectional study. Place and Duration of Study: The Armed Forces Institute of Pathology (AFIP), Rawalpindi, from April to June 2010. Methodology: A total of 60 Leishman stained blood films with clinical suspicion of malaria were studied by light microscopy for detection of malaria parasite (MP). The samples were also subjected to real time PCR for the small subunit (SSU) rRNA gene of MP found in all the four subspecies of Plasmodium. Real time PCR was done by the Taqman probe method. One sample positive for MP was serially diluted with ABO compatible blood, and light microscopy and real time PCR were performed on all dilutions. Results of light microscopy and real time PCR were compared. Sensitivity and specificity were calculated using PCR as the gold standard. Results: PCR detected MP in 33 samples with sensitivity and specificity of 100% whereas light microscopy could detect MP in 30 samples. Sensitivity and specificity of light microscopy was 90.9% and 100% respectively. In the serially diluted blood sample, MP was visible at 1/16 dilution whereas the PCR showed positive results even at 1/512 dilution. onclusion: Real time PCR is more sensitive than light microscopy for the detection of malarial parasite. |
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Authors:
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Jawad Zafar Chaudry; Suhaib Ahmed; Tanveer Zia Qureshi; Nadir Ali |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of the College of Physicians and Surgeons--Pakistan : JCPSP Volume: 22 ISSN: 1681-7168 ISO Abbreviation: J Coll Physicians Surg Pak Publication Date: 2012 Feb |
Date Detail:
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Created Date: 2012-02-08 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9606447 Medline TA: J Coll Physicians Surg Pak Country: Pakistan |
Other Details:
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Languages: eng Pagination: 98-100 Citation Subset: IM |
Affiliation:
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Department of Haematology, Armed Forces Institute of Pathology, Rawalpindi. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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