Document Detail


Real time polymerase chain reaction to diagnose Anaplasma marginale in cattle and deer (Ozotoceros bezoarticus leucogaster) of the Brazilian Pantanal.
MedLine Citation:
PMID:  20943025     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Epizootiological study of Anaplasma marginale in regions that contain various reservoir hosts, co-existence of rickettsia pathogens, and common vectors is a complicated task. To achieve diagnosis of this rickettsia in cattle and campeiro deer of Brazilian Pantanal, a comparison was made between a real time polymerase chain reaction (RT-PCR) with intercalating Sybr Green fluorochrome and primers based on msp5 gene of A. marginale; a conventional PCR (C-PCR); and parasitological examination using thin blood smear stained with Giemsa-MayGrunwald. Both PCRs showed good performance in the diagnosis of A. marginale in cattle, and were superior to the parasitological exam. The RT-PCR detected seven positive campeiro deer (16.3%). This rate was significantly higher compared to C-PCR, which identified one animal as positive (2.3%), and also compared to parasitological diagnosis, which did not find any positive animals. The dissociation temperature average of positive reactions in cattle (81.72 °C ± 0.20) was identical to dissociation temperature found in the cervids (81.72 °C ± 0.12), suggesting that both animal species were infected with A. marginale. We concluded that RT-PCR can be used for A. marginale diagnosis and in epizootiological studies of cattle and cervids; in spite of the small number of campeiro deer samples, the results indicated that this wildlife species has importance in the Anaplasma epizootiology in the Brazilian Pantanal.
Authors:
Graziela Picoloto; Renileide Ferreira de Lima; Lílian Andressa Oliveira Olegário; Cristiano Miranda Espínola Carvalho; Ana Crystina Reis Lacerda; Walfrido Moraes Tomás; Paulo André Lima Borges; Aiesca Oliveira Pellegrin; Cláudio Roberto Madruga
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária     Volume:  19     ISSN:  0103-846X     ISO Abbreviation:  Rev Bras Parasitol Vet     Publication Date:    2010 Jul-Sep
Date Detail:
Created Date:  2010-10-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9440482     Medline TA:  Rev Bras Parasitol Vet     Country:  Brazil    
Other Details:
Languages:  eng     Pagination:  186-8     Citation Subset:  IM    
Affiliation:
Programa de Mestrado em Ciência Animal,Universidade Federal de Mato Grosso do Sul - UFMS, bolsista Fundect,Av. Felinto Muller, 2443, CP 549, CEP 79070-900, Bairro Ipiranga,Campo Grande - MS, Brazil. graziela.picoloto@gmail.com.
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