Document Detail


Real time observation and kinetic modeling of the cellular uptake and removal of silicon quantum dots.
MedLine Citation:
PMID:  22475529     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The time courses of uptake and removal of silicon quantum dots (Si-QDs) by human umbilical endothelial cells (HUVECs) were observed via confocal laser scanning microscope. Si-QDs were internalized via endocytosis and transported to late endosomes/lysosomes. The number of internalized Si-QDs increased with time and gradually reached a plateau value. When Si-QD-internalized HUVECs were subsequently washed and exposed to fresh culture medium, HUVECs removed internalized Si-QDs via exocytosis. The number of internalized Si-QDs decreased with time and gradually reached a plateau value. Not all internalized Si-QDs were removed from the cell interior but large numbers of internalized Si-QDs remained accumulated inside cells. A kinetic model based on the mass balance of Si-QDs and receptors in a cell was proposed to describe the cellular uptake and removal of Si-QDs. Model calculation fitted well with experimental results. Using this model, the dissociation constant between receptors and Si-QDs in the endosome, K(d,in), was found to be a determinant factor for Si-QD accumulation in cells after the removal process.
Authors:
Seiichi Ohta; Susumu Inasawa; Yukio Yamaguchi
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-2
Journal Detail:
Title:  Biomaterials     Volume:  -     ISSN:  1878-5905     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Affiliation:
Department of Chemical System Engineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
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