| Real-time nucleic acid sequence-based amplification using molecular beacons for detection of enterovirus RNA in clinical specimens. | |
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MedLine Citation:
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PMID: 16000425 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Real-time nucleic acid sequence-based amplification (NASBA) using molecular beacon technology (NASBA-beacon) was compared to standard NASBA with postamplification hybridization using electrochemiluminescently labeled probes (NASBA-ECL) for detection of enteroviruses (EV) in 133 cerebrospinal fluid and 27 stool samples. NASBA-ECL and NASBA-beacon were similar in sensitivity, detecting 55 (100%) and 52 (94.5%) EV-positive samples, respectively. There were no false positives. Both NASBA assays were significantly more sensitive than culture. Real-time NASBA-beacon reagents and equipment rental were more expensive than those for NASBA-ECL; however, time to result was shortened by 1.5 h, hands-on time was reduced by 25 min, and the assay was much simpler for technologists to learn and perform. |
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Authors:
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Marie L Landry; Robin Garner; David Ferguson |
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Publication Detail:
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Type: Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of clinical microbiology Volume: 43 ISSN: 0095-1137 ISO Abbreviation: J. Clin. Microbiol. Publication Date: 2005 Jul |
Date Detail:
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Created Date: 2005-07-07 Completed Date: 2005-08-26 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505564 Medline TA: J Clin Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 3136-9 Citation Subset: IM |
Affiliation:
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Department of Laboratory Medicine, P.O. Box 208035, Yale University School of Medicine, New Haven, CT 06520-8035, USA. marie.landry@yale.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Cerebrospinal Fluid / virology Chemiluminescent Measurements / methods Child Electrochemistry / methods Enterovirus / genetics, isolation & purification* Enterovirus Infections / virology* Feces / virology Humans Infant Middle Aged Molecular Probes* RNA, Viral / analysis*, isolation & purification Reagent Kits, Diagnostic Self-Sustained Sequence Replication / methods* Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/Molecular Probes; 0/RNA, Viral; 0/Reagent Kits, Diagnostic |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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