| Real-time myocardial contrast echocardiography for assessing perfusion and function in healthy and infarcted wistar rats. | |
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MedLine Citation:
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PMID: 17854980 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Real-time myocardial contrast echocardiography (MCE) is a noninvasive perfusion imaging method, whereas technical and resolution problems impair its application in small animals. Hence, we investigated the feasibility of MCE in experimental cardiovascular set-ups involving healthy and infarcted myocardium in rats. Twenty-five male Wistar rats were examined under volatile anesthesia (2.5% isoflurane) with high-resolution conventional 2-D echocardiography (2DE) and real-time MCE (Sonos 7,500 with 15MHz-transducer, Philips Medical Systems, Andover, MA, USA) in short-axis view. Contrast agent (SonoVue, Bracco, Milan, Italy) was infused as a bolus into a sublingual vein. Background-subtracted contrast signal intensity (SI) was measured off-line in six end-systolic segments and fitted to an exponential curve (gamma variate). Derived peak SI was subsequently calculated and compared with wall motion and common functional measured quantities (left ventricular end-diastolic diameter [LVEDD], area shortening [AS]). Recordings were performed before and 14 days after left anterior descending (LAD) ligature. Infarction induced anterior wall motion abnormalities (WMA) in all animals (16 akinetic, 9 hypokinetic), increased LVEDD (9.1 +/- 0.6 vs. 7.9 +/- 0.6 mm, p < 0.001), reduced AS (36.1 +/- 10.0 vs. 59.5 +/- 4.1%, p < 0.001) and reduced anterior segmental SI (0.4 +/- 0.4 dB akinetic / 1.7 +/- 1.7 dB hypokinetic vs. 15.8 +/- 10.9 dB preinfarct, p < 0.001 / p < 0.001). Segmental SI in normokinetic segments remained unchanged. Area at risk (perfusion defect) correlated well with WMA (r = 0.838). These data confirmed high-resolution real-time MCE as a rational tool for assessing myocardial perfusion of Wistar rats. It may therefore be a useful diagnostic tool for in-vivo cardiovascular research in small animals. |
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Authors:
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Gerald H Wasmeier; Wolfram-H Zimmermann; Nico Schineis; Ivan Melnychenko; Jens-Uwe Voigt; Thomas Eschenhagen; Frank A Flachskampf; Werner G Daniel; Uwe Nixdorff |
Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't Date: 2007-09-14 |
Journal Detail:
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Title: Ultrasound in medicine & biology Volume: 34 ISSN: 0301-5629 ISO Abbreviation: Ultrasound Med Biol Publication Date: 2008 Jan |
Date Detail:
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Created Date: 2007-12-18 Completed Date: 2008-04-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0410553 Medline TA: Ultrasound Med Biol Country: England |
Other Details:
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Languages: eng Pagination: 47-55 Citation Subset: IM |
Affiliation:
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Second Medical Clinic, Friedrich Alexander University, Erlangen-Nuremberg, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Contrast Media Coronary Circulation Feasibility Studies Male Myocardial Infarction / physiopathology, ultrasonography* Observer Variation Phospholipids / diagnostic use Rats Rats, Wistar Reproducibility of Results Sulfur Hexafluoride / diagnostic use Ventricular Function, Left |
| Chemical | |
Reg. No./Substance:
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0/Contrast Media; 0/Phospholipids; 0/contrast agent BR1; 2551-62-4/Sulfur Hexafluoride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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