Document Detail


Real-time, multidimensional in vivo imaging used to investigate blood flow in mouse pancreatic islets.
MedLine Citation:
PMID:  18846254     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The pancreatic islets of Langerhans are highly vascularized micro-organs that play a key role in the regulation of blood glucose homeostasis. The specific arrangement of endocrine cell types in islets suggests a coupling between morphology and function within the islet. Here, we established a line-scanning confocal microscopy approach to examine the relationship between blood flow and islet cell type arrangement by real-time in vivo imaging of intra-islet blood flow in mice. These data were used to reconstruct the in vivo 3D architecture of the islet and time-resolved blood flow patterns throughout the islet vascular bed. The results revealed 2 predominant blood flow patterns in mouse islets: inner-to-outer, in which blood perfuses the core of beta cells before the islet perimeter of non-beta cells, and top-to-bottom, in which blood perfuses the islet from one side to the other regardless of cell type. Our approach included both millisecond temporal resolution and submicron spatial resolution, allowing for real-time imaging of islet blood flow within the living mouse, which has not to our knowledge been attainable by other methods.
Authors:
Lara R Nyman; K Sam Wells; W Steve Head; Michael McCaughey; Eric Ford; Marcela Brissova; David W Piston; Alvin C Powers
Related Documents :
1482604 - Incomplete vascular casting for a scanning electron microscope study of the microcircul...
24947854 - Current knowledge on the transport and fate of spermatozoa in the reproductive tract of...
22419034 - Increased aquaporin-1 and na(+) -k(+) -2cl(-) cotransporter 1 expression in choroid ple...
23875834 - Comparison of hematologic indices and markers of infection in umbilical cord and neonat...
2537784 - Doppler color flow mapping of an invasive mole.
9284464 - Continuous flow-through peritoneal dialysis (cfpd): comparison of efficiency to ipd, tp...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2008-10-09
Journal Detail:
Title:  The Journal of clinical investigation     Volume:  118     ISSN:  0021-9738     ISO Abbreviation:  J. Clin. Invest.     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-11-04     Completed Date:  2009-01-12     Revised Date:  2013-07-15    
Medline Journal Info:
Nlm Unique ID:  7802877     Medline TA:  J Clin Invest     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3790-7     Citation Subset:  AIM; IM    
Affiliation:
Department of Medicine, Vanderbilt University, Nashville, Tennessee, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Diagnostic Imaging / methods*
Hemodynamics*
Imaging, Three-Dimensional / methods*
Islets of Langerhans / blood supply*,  cytology,  metabolism
Mice
Mice, Transgenic
Microscopy, Confocal
Time Factors
Grant Support
ID/Acronym/Agency:
CA68485/CA/NCI NIH HHS; DK07563/DK/NIDDK NIH HHS; DK20593/DK/NIDDK NIH HHS; DK53434/DK/NIDDK NIH HHS; DK58404/DK/NIDDK NIH HHS; DK59637/DK/NIDDK NIH HHS; DK63439/DK/NIDDK NIH HHS; DK66636/DK/NIDDK NIH HHS; DK68764/DK/NIDDK NIH HHS; DK69603/DK/NIDDK NIH HHS; EY08126/EY/NEI NIH HHS; HD15052/HD/NICHD NIH HHS; P60 DK020593/DK/NIDDK NIH HHS; R01 DK068764/DK/NIDDK NIH HHS; R01 DK069603/DK/NIDDK NIH HHS; R21 DK066636/DK/NIDDK NIH HHS; R33 DK066636/DK/NIDDK NIH HHS; RR22620/RR/NCRR NIH HHS
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  NFATc1 in mice represses osteoprotegerin during osteoclastogenesis and dissociates systemic osteopen...
Next Document:  Calsarcin-2 deficiency increases exercise capacity in mice through calcineurin/NFAT activation.