| Real-time, multidimensional in vivo imaging used to investigate blood flow in mouse pancreatic islets. | |
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MedLine Citation:
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PMID: 18846254 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The pancreatic islets of Langerhans are highly vascularized micro-organs that play a key role in the regulation of blood glucose homeostasis. The specific arrangement of endocrine cell types in islets suggests a coupling between morphology and function within the islet. Here, we established a line-scanning confocal microscopy approach to examine the relationship between blood flow and islet cell type arrangement by real-time in vivo imaging of intra-islet blood flow in mice. These data were used to reconstruct the in vivo 3D architecture of the islet and time-resolved blood flow patterns throughout the islet vascular bed. The results revealed 2 predominant blood flow patterns in mouse islets: inner-to-outer, in which blood perfuses the core of beta cells before the islet perimeter of non-beta cells, and top-to-bottom, in which blood perfuses the islet from one side to the other regardless of cell type. Our approach included both millisecond temporal resolution and submicron spatial resolution, allowing for real-time imaging of islet blood flow within the living mouse, which has not to our knowledge been attainable by other methods. |
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Authors:
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Lara R Nyman; K Sam Wells; W Steve Head; Michael McCaughey; Eric Ford; Marcela Brissova; David W Piston; Alvin C Powers |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2008-10-09 |
Journal Detail:
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Title: The Journal of clinical investigation Volume: 118 ISSN: 0021-9738 ISO Abbreviation: J. Clin. Invest. Publication Date: 2008 Nov |
Date Detail:
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Created Date: 2008-11-04 Completed Date: 2009-01-12 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7802877 Medline TA: J Clin Invest Country: United States |
Other Details:
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Languages: eng Pagination: 3790-7 Citation Subset: AIM; IM |
Affiliation:
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Department of Medicine, Vanderbilt University, Nashville, Tennessee, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Diagnostic Imaging / methods* Hemodynamics* Imaging, Three-Dimensional / methods* Islets of Langerhans / blood supply*, cytology, metabolism Mice Mice, Transgenic Microscopy, Confocal Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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CA68485/CA/NCI NIH HHS; DK07563/DK/NIDDK NIH HHS; DK20593/DK/NIDDK NIH HHS; DK53434/DK/NIDDK NIH HHS; DK58404/DK/NIDDK NIH HHS; DK59637/DK/NIDDK NIH HHS; DK63439/DK/NIDDK NIH HHS; DK66636/DK/NIDDK NIH HHS; DK68764/DK/NIDDK NIH HHS; DK69603/DK/NIDDK NIH HHS; EY08126/EY/NEI NIH HHS; HD15052/HD/NICHD NIH HHS; RR22620/RR/NCRR NIH HHS |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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