| Real time monitoring of multiple parameters in mammalian cell culture bioreactors using an in-line Raman spectroscopy probe. | |
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MedLine Citation:
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PMID: 21449033 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The FDA's process analytical technology initiative encourages drug manufacturers to apply innovative ideas to better understand their processes. There are many challenges to applying these techniques to monitor mammalian cell culture bioreactors for biologics manufacturing. These include the ability to monitor multiple components in complex medium formulations non-invasively and in-line. We report results that demonstrate, for the first time, the technical feasibility of the in-line application of Raman spectroscopy for monitoring a mammalian cell culture bioreactor. A Raman probe was used for the simultaneous prediction of culture parameters including glutamine, glutamate, glucose, lactate, ammonium, viable cell density, and total cell density. Bioeng. 2011; 108:1215-1221. © 2010 Wiley Periodicals, Inc. |
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Authors:
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Nicholas R Abu-Absi; Brian M Kenty; Maryann Ehly Cuellar; Michael C Borys; Sivakesava Sakhamuri; David J Strachan; Michael C Hausladen; Zheng Jian Li |
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Publication Detail:
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Type: Letter Date: 2010-12-22 |
Journal Detail:
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Title: Biotechnology and bioengineering Volume: 108 ISSN: 1097-0290 ISO Abbreviation: Biotechnol. Bioeng. Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-03-30 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7502021 Medline TA: Biotechnol Bioeng Country: United States |
Other Details:
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Languages: eng Pagination: 1215-21 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Wiley Periodicals, Inc. |
Affiliation:
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Process Sciences, Worldwide Medicines Group, Bristol-Myers Squibb Company, P.O. Box 4755, Syracuse, New York 13221; telephone: +1-315-432-2131; fax: +1-315-432-2341. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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