Document Detail


Real-time monitoring of cell-free protein synthesis on a surface plasmon resonance chip.
MedLine Citation:
PMID:  17543877     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Taking advantage of the "open" nature of cell-free protein synthesis, this study investigated the direct analysis of protein expression using a surface plasmon resonance sensor. During the on-chip incubation of the reaction mixture for cell-free protein synthesis, the expressed protein molecules were immobilized onto the surface of the chip, giving rise to a sensorgram signal, which enabled on-line monitoring of protein expression. In addition, we found that the expression of the aggregation-prone proteins could be effectively monitored. The ability to monitor these proteins was most likely through the instant isolation of the expressed protein molecules onto the solid surface of the chip.
Authors:
Kyung-Ho Lee; Hyou-Arm Joung; Jin-Ho Ahn; Kyeong-Ohn Kim; In-Seok Oh; Yong-Beom Shin; Min-Gon Kim; Dong-Myung Kim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-05-03
Journal Detail:
Title:  Analytical biochemistry     Volume:  366     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-06-18     Completed Date:  2007-08-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  170-4     Citation Subset:  IM    
Affiliation:
Department of Fine Chemical Engineering and Chemistry, Chungnam National University, Daejeon 305-764, Korea.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / genetics,  metabolism
Escherichia coli / genetics,  metabolism*
Green Fluorescent Proteins / genetics,  metabolism
Protein Biosynthesis*
Reproducibility of Results
Surface Plasmon Resonance / instrumentation,  methods*
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 147336-22-9/Green Fluorescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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