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Real-time monitoring of adherent Vero cell density and apoptosis in bioreactor processes.
MedLine Citation:
PMID:  22367019     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
This study proposes an easy to use in situ device, based on multi-frequency permittivity measurements, to monitor the growth and death of attached Vero cells cultivated on microporous microcarriers, without any cell sampling. Vero cell densities were on-line quantified up to 10(6) cell mL(-1). Some parameters which could potentially impact Vero cell morphological and physiological states were assessed through different culture operating conditions, such as media formulation or medium feed-harvest during cell growth phase. A new method of in situ cell death detection with dielectric spectroscopy was also successfully implemented. Thus, through permittivity frequency scanning, major rises of the apoptotic cell population in bioreactor cultures were detected by monitoring the characteristic frequency of the cell population, f(c), which is one of the culture dielectric parameters. Both cell density quantification and cell apoptosis detection are strategic information in cell-based production processes as they are involved in major events of the process, such as scale-up or choice of the viral infection conditions. This new application of dielectric spectroscopy to adherent cell culture processes makes it a very promising tool for risk-mitigation strategy in industrial processes. Therefore, our results contribute to the development of Process Analytical Technology in cell-based industrial processes.
Authors:
Emma Petiot; Amal El-Wajgali; Geoffrey Esteban; Cécile Gény; Hervé Pinton; Annie Marc
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-2-25
Journal Detail:
Title:  Cytotechnology     Volume:  -     ISSN:  0920-9069     ISO Abbreviation:  -     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-2-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8807027     Medline TA:  Cytotechnology     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Laboratoire Réactions et Génie des Procédés, UPR CNRS 3349, Nancy-Université, 2 avenue de la Forêt de Haye, 54505, Vandoeuvre-lès-Nancy Cedex, France, Emma.Petiot@cnrc-nrc.gc.ca.
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