Document Detail

Real-time microgravimetric quantification of Cryptosporidium parvum in the presence of potential interferents.
MedLine Citation:
PMID:  19375770     Owner:  NLM     Status:  MEDLINE    
The quartz crystal microbalance with dissipation monitoring (QCM-D) is used to develop a biosensor for detection of viable Cryptosporidium parvum (C. parvum) in water matrices of varying complexity. In a clean environment, a good log-log linear response is obtained for detection of C. parvum in aqueous suspensions with oocyst concentrations from 3x10(5) to 1x10(7)oocysts/mL. C. parvum detection is slightly affected by the presence of dissolved organic acids, likely due to steric stabilization and/or masking of the antibodies/antigens by adsorbed molecules. Colloidal contaminants generally have a greater influence as biosensor interferents, whereby the presence of model latex microspheres, Enterococcus faecalis, or Escherichia coli, led to decreases in biosensor response of up to 64%, 40%, and 20%, respectively.
Charles Poitras; Julien Fatisson; Nathalie Tufenkji
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-03-21
Journal Detail:
Title:  Water research     Volume:  43     ISSN:  0043-1354     ISO Abbreviation:  Water Res.     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-05-22     Completed Date:  2009-07-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0105072     Medline TA:  Water Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  2631-8     Citation Subset:  IM    
Department of Chemical Engineering, McGill University, Montreal, Quebec, Canada H3A 2B2.
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MeSH Terms
Biosensing Techniques*
Cryptosporidium parvum / isolation & purification*,  ultrastructure
Enterococcus faecalis
Escherichia coli
Micro-Electrical-Mechanical Systems / methods*
Microscopy, Atomic Force
Microscopy, Electron, Scanning
Oocysts / ultrastructure

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