| Real-time microgravimetric quantification of Cryptosporidium parvum in the presence of potential interferents. | |
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MedLine Citation:
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PMID: 19375770 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The quartz crystal microbalance with dissipation monitoring (QCM-D) is used to develop a biosensor for detection of viable Cryptosporidium parvum (C. parvum) in water matrices of varying complexity. In a clean environment, a good log-log linear response is obtained for detection of C. parvum in aqueous suspensions with oocyst concentrations from 3x10(5) to 1x10(7)oocysts/mL. C. parvum detection is slightly affected by the presence of dissolved organic acids, likely due to steric stabilization and/or masking of the antibodies/antigens by adsorbed molecules. Colloidal contaminants generally have a greater influence as biosensor interferents, whereby the presence of model latex microspheres, Enterococcus faecalis, or Escherichia coli, led to decreases in biosensor response of up to 64%, 40%, and 20%, respectively. |
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Authors:
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Charles Poitras; Julien Fatisson; Nathalie Tufenkji |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-03-21 |
Journal Detail:
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Title: Water research Volume: 43 ISSN: 0043-1354 ISO Abbreviation: Water Res. Publication Date: 2009 Jun |
Date Detail:
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Created Date: 2009-05-22 Completed Date: 2009-07-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0105072 Medline TA: Water Res Country: England |
Other Details:
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Languages: eng Pagination: 2631-8 Citation Subset: IM |
Affiliation:
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Department of Chemical Engineering, McGill University, Montreal, Quebec, Canada H3A 2B2. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biosensing Techniques* Cryptosporidium parvum / isolation & purification*, ultrastructure Enterococcus faecalis Escherichia coli Micro-Electrical-Mechanical Systems / methods* Microscopy, Atomic Force Microscopy, Electron, Scanning Oocysts / ultrastructure |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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