| Real-time measurements of coagulation on bacterial cellulose and conventional vascular graft materials. | |
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MedLine Citation:
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PMID: 19800035 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The search for a functional, small diameter (<5mm) vascular graft has been ongoing for over 30 years, but yet there is no consistently reliable synthetic graft. The primary mechanisms of graft failure are intimal hyperplasia, poor blood flow and surface thrombogenicity. Bacterial cellulose (BC) became therefore a proposed new biosynthetic vascular graft material. Since conventional methods are not suited for coagulation measurements on BC, we have adapted the automated calibrated thrombin generation method for measurements of biomaterial-induced coagulation of BC as compared with clinically used graft materials i.e., expanded poly(tetrafluoroethylene) (ePTFE) and poly(ethyleneterephtalat) (PET). We have also visualized the coagulation propagation at the material surfaces. Thrombin generation experiments revealed dramatic differences between the materials tested. Both ePTFE and BC were found to generate longer lag times and ttpeak values than PET. Most importantly, BC was found to generate the lowest "peak", indicating a slower coagulation process at the surface. These results are also supported by the measurements of factor XIIa generation and analysis of surface coagulation times, which were detected in the following increasing order (mean + or - SD): PET (27 + or - 8 min)<BC (46 + or - 9 min)<ePTFE (61 + or - 21 min). Real-time measurement of coagulation seems to have the potential for becoming a powerful tool for evaluation of biomaterials for blood-contacting devices. |
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Authors:
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Helen Fink; Lars Fax?lv; G?bor F Moln?r; Kristoffer Drotz; Bo Risberg; Tomas L Lindahl; Anders Sellborn |
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Publication Detail:
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Type: Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't Date: 2009-09-30 |
Journal Detail:
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Title: Acta biomaterialia Volume: 6 ISSN: 1878-7568 ISO Abbreviation: Acta Biomater Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-02-01 Completed Date: 2010-03-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101233144 Medline TA: Acta Biomater Country: England |
Other Details:
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Languages: eng Pagination: 1125-30 Citation Subset: IM |
Copyright Information:
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Copyright 2009 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved. |
Affiliation:
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Department of Clinical Sciences, Vascular Engineering Centre, Sahlgrenska Academy at University of Gothenburg, SE-41345 G?teborg, Sweden. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biocompatible Materials
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chemistry* Blood Coagulation / physiology* Blood Vessel Prosthesis* Cellulose / chemistry* Computer Systems Gluconacetobacter xylinus / metabolism* Humans Materials Testing / methods Transplants* |
| Chemical | |
Reg. No./Substance:
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0/Biocompatible Materials; 9004-34-6/Cellulose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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