Document Detail


Real-time full-spectral imaging and affinity measurements from 50 microfluidic channels using nanohole surface plasmon resonance.
MedLine Citation:
PMID:  22895607     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
With recent advances in high-throughput proteomics and systems biology, there is a growing demand for new instruments that can precisely quantify a wide range of receptor-ligand binding kinetics in a high-throughput fashion. Here we demonstrate a surface plasmon resonance (SPR) imaging spectroscopy instrument capable of simultaneously extracting binding kinetics and affinities from 50 parallel microfluidic channels. The instrument utilizes large-area (~ cm(2)) metallic nanohole arrays as SPR sensing substrates and combines a broadband light source, a high-resolution imaging spectrometer and a low-noise CCD camera to extract spectral information from every channel in real time with a refractive index resolution of 7.7 × 10(-6) refractive index units. To demonstrate the utility of our instrument for quantifying a wide range of biomolecular interactions, each parallel microfluidic channel is coated with a biomimetic supported lipid membrane containing ganglioside (GM1) receptors. The binding kinetics of cholera toxin b (CTX-b) to GM1 are then measured in a single experiment from 50 channels. By combining the highly parallel microfluidic device with large-area periodic nanohole array chips, our SPR imaging spectrometer system enables high-throughput, label-free, real-time SPR biosensing, and its full-spectral imaging capability combined with nanohole arrays could enable integration of SPR imaging with concurrent surface-enhanced Raman spectroscopy.
Authors:
Si Hoon Lee; Nathan C Lindquist; Nathan J Wittenberg; Luke R Jordan; Sang-Hyun Oh
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Lab on a chip     Volume:  12     ISSN:  1473-0189     ISO Abbreviation:  Lab Chip     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-19     Completed Date:  2013-02-14     Revised Date:  2013-10-22    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  England    
Other Details:
Languages:  eng     Pagination:  3882-90     Citation Subset:  IM    
Affiliation:
Laboratory of Nanostructures and Biosensing, Department of Electrical and Computer Engineering, University of Minnesota, Minneapolis, MN 55455, USA.
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MeSH Terms
Descriptor/Qualifier:
Biomimetic Materials / chemistry
Cholera Toxin / chemistry*
G(M1) Ganglioside / chemistry*
Membranes, Artificial
Microfluidic Analytical Techniques / instrumentation*,  methods*
Surface Plasmon Resonance / instrumentation*,  methods*
Grant Support
ID/Acronym/Agency:
R01 GM 095638/GM/NIGMS NIH HHS; R01 GM092993/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Membranes, Artificial; 37758-47-7/G(M1) Ganglioside; 9012-63-9/Cholera Toxin
Comments/Corrections

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