Document Detail

Real-time dynamics of label-free single mast cell granules revealed by differential interference contrast microscopy.
MedLine Citation:
PMID:  16633786     Owner:  NLM     Status:  MEDLINE    
We demonstrate the capability of differential interference contrast (DIC) microscopy as a simple and useful tool for studying cellular events without fluorescence labeling. By coupling an advanced DIC microscope to a computer-controlled motorized vertical stage and a high-speed, high-resolution CCD camera, real-time three-dimensional monitoring is possible in a high-throughput manner. The performance among three modes of microscopy, bright-field, dark-field and DIC, in terms of horizontal resolving power and vertical sectioning was investigated. As a model, exocytosis of rat peritoneal mast cells was recorded on the subsecond time scale. Three-dimensional tracking of granules during degranulation was achieved and granule-granule fusion before plasma membrane fusion was recorded.
Hung-Wing Li; Michael McCloskey; Yan He; Edward S Yeung
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2006-04-22
Journal Detail:
Title:  Analytical and bioanalytical chemistry     Volume:  387     ISSN:  1618-2642     ISO Abbreviation:  Anal Bioanal Chem     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2006-12-20     Completed Date:  2007-06-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101134327     Medline TA:  Anal Bioanal Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  63-9     Citation Subset:  IM    
Ames Laboratory-USDOE and Department of Chemistry, Iowa State University, Ames, IA 50011, USA.
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MeSH Terms
Cell Degranulation / physiology*
Concanavalin A / pharmacology
Cytoplasmic Granules / physiology
Immunoglobulin E / pharmacology
Mast Cells / cytology,  drug effects,  physiology*
Membrane Fusion / physiology
Microscopy, Interference / methods*
Polymyxin B / pharmacology
Rats, Wistar
Reg. No./Substance:
11028-71-0/Concanavalin A; 1404-26-8/Polymyxin B; 37341-29-0/Immunoglobulin E

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