| Real-time dynamics of label-free single mast cell granules revealed by differential interference contrast microscopy. | |
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MedLine Citation:
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PMID: 16633786 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We demonstrate the capability of differential interference contrast (DIC) microscopy as a simple and useful tool for studying cellular events without fluorescence labeling. By coupling an advanced DIC microscope to a computer-controlled motorized vertical stage and a high-speed, high-resolution CCD camera, real-time three-dimensional monitoring is possible in a high-throughput manner. The performance among three modes of microscopy, bright-field, dark-field and DIC, in terms of horizontal resolving power and vertical sectioning was investigated. As a model, exocytosis of rat peritoneal mast cells was recorded on the subsecond time scale. Three-dimensional tracking of granules during degranulation was achieved and granule-granule fusion before plasma membrane fusion was recorded. |
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Authors:
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Hung-Wing Li; Michael McCloskey; Yan He; Edward S Yeung |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2006-04-22 |
Journal Detail:
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Title: Analytical and bioanalytical chemistry Volume: 387 ISSN: 1618-2642 ISO Abbreviation: Anal Bioanal Chem Publication Date: 2007 Jan |
Date Detail:
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Created Date: 2006-12-20 Completed Date: 2007-06-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101134327 Medline TA: Anal Bioanal Chem Country: Germany |
Other Details:
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Languages: eng Pagination: 63-9 Citation Subset: IM |
Affiliation:
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Ames Laboratory-USDOE and Department of Chemistry, Iowa State University, Ames, IA 50011, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Degranulation / physiology* Concanavalin A / pharmacology Cytoplasmic Granules / physiology Female Immunoglobulin E / pharmacology Mast Cells / cytology, drug effects, physiology* Membrane Fusion / physiology Microscopy, Interference / methods* Polymyxin B / pharmacology Rats Rats, Wistar |
| Chemical | |
Reg. No./Substance:
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11028-71-0/Concanavalin A; 1404-26-8/Polymyxin B; 37341-29-0/Immunoglobulin E |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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