| Real-time assays with molecular beacons and other fluorescent nucleic acid hybridization probes. | |
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MedLine Citation:
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PMID: 16111667 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: A number of formats for nucleic acid hybridization have been developed to identify DNA and RNA sequences that are involved in cellular processes and that aid in the diagnosis of genetic and infectious diseases. METHODS: The introduction of hybridization probes with interactive fluorophore pairs has enabled the development of homogeneous hybridization assays for the direct identification of nucleic acids. A change in the fluorescence of these probes indicates the presence of a target nucleic acid, and there is no need to separate unbound probes from hybridized probes. CONCLUSIONS: The advantages of homogeneous hybridization assays are their speed and simplicity. In addition, homogeneous assays can be combined with nucleic acid amplification, enabling the detection of rare target nucleic acids. These assays can be followed in real time, providing quantitative determination of target nucleic acids over a broad range of concentrations. |
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Authors:
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Salvatore A E Marras; Sanjay Tyagi; Fred Russell Kramer |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Review Date: 2005-08-18 |
Journal Detail:
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Title: Clinica chimica acta; international journal of clinical chemistry Volume: 363 ISSN: 0009-8981 ISO Abbreviation: Clin. Chim. Acta Publication Date: 2006 Jan |
Date Detail:
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Created Date: 2005-12-06 Completed Date: 2006-03-02 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 1302422 Medline TA: Clin Chim Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 48-60 Citation Subset: IM |
Affiliation:
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Department of Molecular Genetics, Public Health Research Institute, Newark, NJ 07103, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Assay / methods*, trends DNA / metabolism Fluorescent Dyes / chemistry*, metabolism Gene Amplification Humans Molecular Probe Techniques* Nucleic Acid Hybridization / methods* Oligonucleotide Probes / chemistry*, metabolism RNA / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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AI-056689/AI/NIAID NIH HHS; EB-000277/EB/NIBIB NIH HHS; GM-070357/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 0/Oligonucleotide Probes; 63231-63-0/RNA; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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