| Real time PCR profiling of 330 human micro-RNAs. | |
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MedLine Citation:
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PMID: 17124719 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The small size of miRNAs makes profiling of all the 462 known human miRNAs difficult using single cell samples. Recently, we showed that judicious sequence partitioning between RT primers and second strand synthesis primers permitted multiplexed RT-PCR amplification of miRNA in very small samples to allow individual real time PCR quantification. Here, we show that zip coding the primers and TaqMan probes with sequences specific to each miRNA greatly improves reaction specificity, which permits the profiling of all miRNAs in a single multiplexed RT-PCR reaction. |
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Authors:
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Kaiqin Lao; Nan Lan Xu; Yongming Andrew Sun; Kenneth J Livak; Neil A Straus |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Technical Report |
Journal Detail:
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Title: Biotechnology journal Volume: 2 ISSN: 1860-7314 ISO Abbreviation: Biotechnol J Publication Date: 2007 Jan |
Date Detail:
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Created Date: 2007-01-22 Completed Date: 2007-02-21 Revised Date: 2007-06-12 |
Medline Journal Info:
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Nlm Unique ID: 101265833 Medline TA: Biotechnol J Country: Germany |
Other Details:
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Languages: eng Pagination: 33-5 Citation Subset: IM |
Affiliation:
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Applied Biosystems, Foster City, CA, USA. laokq@appliedbiosystems.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Computer Systems Humans MicroRNAs / genetics* Molecular Sequence Data RNA Probes / genetics* Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction / methods* Sensitivity and Specificity Sequence Analysis, RNA / methods* |
| Chemical | |
Reg. No./Substance:
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0/MicroRNAs; 0/RNA Probes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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