Document Detail


Real-time PCR detection of salmonella in suspect foods from a gastroenteritis outbreak in kerr county, Texas.
MedLine Citation:
PMID:  12149377     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In June 2001, an outbreak of acute gastroenteritis among 109 attendees of a church picnic in Kerr County, Texas, was reported. A 5'-nuclease PCR assay was used to screen for Salmonella in nine food items from the buffet line. Barbeque chicken B tested positive for Salmonella, and no amplification was detected in the remaining food items. These PCR findings were consistent with culture results and were confirmed by direct nucleotide sequencing. Salmonella enterica serotype Panama was cultured from both food and patient stool samples.
Authors:
Luke T Daum; William J Barnes; James C McAvin; Margaret S Neidert; Lynn A Cooper; William B Huff; Linda Gaul; W S Riggins; Sandra Morris; Ann Salmen; Kenton L Lohman
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  40     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-07-31     Completed Date:  2002-10-30     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3050-2     Citation Subset:  IM    
Affiliation:
Brooks Air Force Base, San Antonio, Texas 78235-5237, USA. Luke.Daum@brooks.af.mil
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Typing Techniques
Cattle
DNA, Bacterial / analysis
Disease Outbreaks*
Feces / microbiology
Food Microbiology
Gastroenteritis / epidemiology*,  microbiology
Humans
Meat / microbiology*
Polymerase Chain Reaction / methods*
Salmonella Food Poisoning / epidemiology*,  microbiology
Salmonella enterica / classification*,  genetics,  isolation & purification
Texas / epidemiology
Chemical
Reg. No./Substance:
0/DNA, Bacterial
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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