Document Detail


Real-time PCR for the detection of Salmonella spp. in food: An alternative approach to a conventional PCR system suggested by the FOOD-PCR project.
MedLine Citation:
PMID:  16564585     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A real-time PCR assay using non-patented primers and a TaqMan probe for the detection and quantification of Salmonella spp. is presented. The assay is based on an internationally validated conventional PCR system, which was suggested as a standard method for the detection of Salmonella spp. in the FOOD-PCR project. The assay was sensitive and specific. Consistent detection of 9.5 genome equivalents per PCR reaction was achieved, whereas samples containing an average of 0.95 genome equivalents per reaction were inconsistently positive. The assay performed equally well as a commercially available real-time PCR assay and allowed sensitive detection of Salmonella spp. in artificially contaminated food. After enrichment for 16 h in buffered peptone water (BPW) or universal pre-enrichment broth (UPB) 2.5 CFU/25 g salmon and minced meat, and 5 CFU/25 g chicken meat and 25 ml raw milk were detected. Enrichment in BPW yielded higher numbers of CFU/ml than UPB for all matrices tested. However, the productivity of UPB was sufficient, as all samples were positive with both real-time PCR methods, including those containing less than 300 CFU/ml enrichment broth (enrichment of 5 CFU/25 ml raw milk in UPB).
Authors:
Ingeborg Hein; Gabriele Flekna; Martina Krassnig; Martin Wagner
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-03-06
Journal Detail:
Title:  Journal of microbiological methods     Volume:  66     ISSN:  0167-7012     ISO Abbreviation:  J. Microbiol. Methods     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-07-21     Completed Date:  2006-09-26     Revised Date:  2007-06-20    
Medline Journal Info:
Nlm Unique ID:  8306883     Medline TA:  J Microbiol Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  538-47     Citation Subset:  IM    
Affiliation:
Institute of Milk Hygiene, Milk Technology, and Food Science, Department of Veterinary Public Health and Food Science, University of Veterinary Medicine, Veterinaerplatz 1, A-1210 Vienna, Austria. ingeborg.hein@vu-wien.ac.at
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Chickens
Culture Media
DNA, Bacterial / chemistry,  genetics
Food Microbiology*
Meat / microbiology*
Milk / microbiology*
Polymerase Chain Reaction
Salmon
Salmonella / genetics,  isolation & purification*
Salmonella Food Poisoning / microbiology*,  prevention & control
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Culture Media; 0/DNA, Bacterial
Comments/Corrections
Erratum In:
J Microbiol Methods. 2007 Jun;69(3):533

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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