Document Detail

Real-time PCR detection of Pseudomonas aeruginosa in clinical and municipal wastewater and genotyping of the ciprofloxacin-resistant isolates.
MedLine Citation:
PMID:  16819959     Owner:  NLM     Status:  MEDLINE    
Real-time quantification of Pseudomonas aeruginosa was performed in various wastewater systems including clinical, municipal wastewaters and inflow from a wastewater treatment plant. The highest concentrations of P. aeruginosa-specific targets were detected in clinical wastewaters. Limitations of the detection system resulting from inhibition or cross-reaction were identified. Ciprofloxacin-resistant P. aeruginosa strains were isolated after specific enrichment from clinical and municipal wastewaters. In some cases they were also cultivated from effluent of a wastewater treatment plant, and from its downstream river water. A total of 119 isolates were phenotypically characterized as ciprofloxacin-resistant via antibiogram testing. Subsequently, the fluoroquinolone-resistance-mediating mutations in the genes gyrA codon positions 83 and 87, gyrB codon position 466 and parC codon positions 87 and 91 were determined by mini-sequencing. Ciprofloxacin resistance was mainly associated with mutations in gyrA codon position 83 and parC mutation in codon positions 87 or 91 of the bacterial gyrase and topoisomerase II genes. All ciprofloxacin-resistant P. aeruginosa strains were compared with genotypes from clinical data of fluoroquinolone-resistant P. aeruginosa infections. The results were in agreement with data from clinical analyses, with the exception that no gyrA 87 and no gyrB mutations were found in ciprofloxacin-resistant P. aeruginosa wastewater isolates.
Thomas Schwartz; Holger Volkmann; Silke Kirchen; Wolfgang Kohnen; Katja Schön-Hölz; Bernd Jansen; Ursula Obst
Related Documents :
9311899 - Partial resistance to gd-mediated interference conferred by mutations affecting herpes ...
15127369 - Quinolone resistance mechanisms in pneumococci.
7840589 - Gyra mutations in quinolone-resistant isolates of the fish pathogen aeromonas salmonicida.
8737139 - Mutations responsible for reduced susceptibility to 4-quinolones in clinical isolates o...
5321479 - Kinetic and genetic analyses of d-cycloserine inhibition and resistance in escherichia ...
3026239 - Purification and properties of dna gyrase from a fluoroquinolone-resistant strain of es...
21605279 - Clinical and molecular microbiological characteristics of carbapenem-resistant acinetob...
19868829 - Studies on virulence : ii. the increase in virulence in vitro of a strain of pneumococcus.
21235549 - Spawning substrata are important for breeding habitat selection but do not determine pr...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEMS microbiology ecology     Volume:  57     ISSN:  0168-6496     ISO Abbreviation:  FEMS Microbiol. Ecol.     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-07-05     Completed Date:  2006-10-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8901229     Medline TA:  FEMS Microbiol Ecol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  158-67     Citation Subset:  IM    
Forschungszentrum Karlsruhe, Department of Environmental Microbiology: Water Technology and Geotechnology Division, Institute of Technical Chemistry, Karlsruhe, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Ciprofloxacin / metabolism*
Drug Resistance, Bacterial / genetics*
Fluoroquinolones / metabolism*
Medical Waste Disposal
Molecular Sequence Data
Polymerase Chain Reaction / methods
Pseudomonas aeruginosa / genetics,  isolation & purification*
RNA, Ribosomal, 23S / genetics
Sewage / microbiology*
Reg. No./Substance:
0/Fluoroquinolones; 0/Medical Waste Disposal; 0/RNA, Ribosomal, 23S; 0/Sewage; 85721-33-1/Ciprofloxacin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Quantification of mcrA by quantitative fluorescent PCR in sediments from methane seep of the Nankai ...
Next Document:  Mammalian PGRPs: novel antibacterial proteins.