Document Detail


Real-time PCR assay for rapid detection and quantification of Campylobacter jejuni on chicken rinses from poultry processing plant.
MedLine Citation:
PMID:  17223308     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Campylobacter jejuni (C. jejuni) is the leading cause of food-borne gastroenteritis in the United States. Detection of Campylobacter in food samples by conventional culture is cumbersome; therefore, there is a need to develop rapid and cost-effective detection and quantification methods. Eighty-four whole chicken rinses were collected at different stages of processing at three poultry processing plants. After chicken wash collection and DNA extraction, the samples were directly subjected to real-time PCR (rtPCR) without enrichment and also culture. The assay specificity was determined with a range of Campylobacter species, related, and unrelated organisms. Of the 84 samples collected 65 (77%) of the samples were positive by the rtPCR assay and 27 (32%) of the samples tested positive by direct plating to selective agar media. The results were positively concordant for 27 (32%) of the samples. The whole rtPCR assay can be completed within 90min with a detection limit of 1CFU, compared to 5-7 days for enrichment and sub culturing in selective agar. This assay is the first report of rtPCR method capable of detecting and quantifying C. jejuni from chicken rinses without an enrichment step and could be an important, rapid and quantification model for other food-borne pathogens.
Authors:
Aradom Debretsion; Tsegaye Habtemariam; Saul Wilson; David Nganwa; Teshome Yehualaeshet
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Publication Detail:
Type:  Evaluation Studies; Journal Article     Date:  2006-11-14
Journal Detail:
Title:  Molecular and cellular probes     Volume:  21     ISSN:  0890-8508     ISO Abbreviation:  Mol. Cell. Probes     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-03-19     Completed Date:  2007-06-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8709751     Medline TA:  Mol Cell Probes     Country:  England    
Other Details:
Languages:  eng     Pagination:  177-81     Citation Subset:  IM    
Affiliation:
Department of Pathobiology, College of Veterinary Medicine, Nursing & Allied Health, Tuskegee University, AL 36088, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Calibration
Campylobacter jejuni / genetics,  isolation & purification*
Chickens / microbiology*
Computer Systems
Food Handling*
Industrial Waste / analysis
Polymerase Chain Reaction / methods*
Reference Values
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Industrial Waste

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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