Document Detail


Real-time PCR assay in Leishmania-infected dogs treated with meglumine antimoniate and allopurinol.
MedLine Citation:
PMID:  17553711     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A real-time PCR assay was exploited for monitoring the Leishmania DNA load in different tissues from 18 naturally-infected dogs before and after treatment with a combination of meglumine antimoniate (100mg/kg/day, subcutaneously) and allopurinol (10mg/kg/day, orally) for 30 days. After the combined therapy, allopurinol was continued at the same dose until the end of the observation period. Whole blood samples, lymph node aspirates, and skin biopsies were collected at the time of diagnosis, 1 month after starting therapy, and every 3 months for 2 years. In six dogs parasite load assessments continued every 6 months for a further 3 years. At each assessment, the dogs were examined for signs of disease and a clinical score was recorded. At diagnosis, the highest Leishmania DNA load was detected in lymph node aspirates. From 1-6 months post-therapy a general improvement in clinical conditions was recorded in all dogs, which correlated with a decrease in the parasite DNA load in all tested tissues, even though it was less pronounced in lymph node aspirates. In the period from 9-24 months post-therapy, a re-increase in parasite load was observed in the tissues of some dogs, concomitant with a disease relapse. The results show that the combined therapy with meglumine antimoniate and allopurinol promoted a clinical improvement which was accompanied by a reduction in the parasitic load in the blood, skin and lymph nodes but, even after long period of allopurinol administration alone, Leishmania may persist in dog tissues.
Authors:
Laura Manna; Stefano Reale; Fabrizio Vitale; Esther Picillo; Luigi Michele Pavone; Angelo Elio Gravino
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-06-05
Journal Detail:
Title:  Veterinary journal (London, England : 1997)     Volume:  177     ISSN:  1090-0233     ISO Abbreviation:  Vet. J.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-06-16     Completed Date:  2008-10-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706281     Medline TA:  Vet J     Country:  England    
Other Details:
Languages:  eng     Pagination:  279-82     Citation Subset:  IM    
Affiliation:
Dipartimento di Scienze Cliniche Veterinarie, Università di Napoli Federico II, Napoli, Italy. laumanna@unina.it
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MeSH Terms
Descriptor/Qualifier:
Allopurinol / administration & dosage,  therapeutic use*
Animals
Antiprotozoal Agents / therapeutic use*
DNA, Protozoan / isolation & purification
Dog Diseases / drug therapy*
Dogs
Drug Therapy, Combination
Leishmania / isolation & purification
Leishmaniasis / drug therapy,  veterinary*
Lymph Nodes / parasitology
Meglumine / administration & dosage,  therapeutic use*
Organometallic Compounds / administration & dosage,  therapeutic use*
Polymerase Chain Reaction / veterinary*
Skin / parasitology
Chemical
Reg. No./Substance:
0/Antiprotozoal Agents; 0/DNA, Protozoan; 0/Organometallic Compounds; 133-51-7/meglumine antimoniate; 315-30-0/Allopurinol; 6284-40-8/Meglumine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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