| Real-time PCR of the 16S-rRNA gene in the diagnosis of neonatal bacteraemia. | |
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MedLine Citation:
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PMID: 18624992 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To evaluate a real-time PCR assay for the diagnosis of neonatal bacteraemia. PATIENTS AND METHODS: Two hundred ninety-five plasma samples from 288 newborns with suspected neonatal sepsis were collected prospectively for the purpose of polymerase chain reaction (PCR)-based bacterial detection. A real-time PCR targeting the bacterial gene for 16S-rRNA gene combined with four specific probes designed to detect Gram-negative bacteria, Staphylococcus aureus and coagulase-negative staphylococci (CoNS) was developed. All samples positive in the universal PCR were further sequenced for bacterial identification. RESULTS: When applied to a material from 50 patients with positive blood culture and 245 patients with negative blood culture, the universal PCR showed a sensitivity of 42% (28-57), a specificity of 95% (92-97), a positive predictive value of 64% (45-80), and a negative predictive value of 89% (84-92) (95% confidence intervals in brackets). CONCLUSION: A new real-time PCR technique was for the first time applied to a well-defined prospectively and consecutively enrolled material of newborns with suspected sepsis, combining the benefits of real-time PCR with specific probes and sequencing. The method managed to detect bacteraemia with high specificity even though the sensitivity was low. Factors causing the low sensitivity are identified and further strategies to develop the method are described. |
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Authors:
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Andreas Ohlin; Anders Bäckman; Maria Björkqvist; Paula Mölling; Margaretha Jurstrand; Jens Schollin |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-07-09 |
Journal Detail:
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Title: Acta paediatrica (Oslo, Norway : 1992) Volume: 97 ISSN: 0803-5253 ISO Abbreviation: Acta Paediatr. Publication Date: 2008 Oct |
Date Detail:
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Created Date: 2008-09-10 Completed Date: 2009-01-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9205968 Medline TA: Acta Paediatr Country: Norway |
Other Details:
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Languages: eng Pagination: 1376-80 Citation Subset: IM |
Affiliation:
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Department of Paediatrics, Orebro University Hospital, Orebro, Sweden. andreas.ohlin@orebroll.se |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacteremia
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diagnosis*,
genetics* Base Sequence Diagnostic Tests, Routine Humans Infant, Newborn Pilot Projects RNA Probes / diagnostic use RNA, Ribosomal, 16S / analysis* Reverse Transcriptase Polymerase Chain Reaction* Risk Assessment Sensitivity and Specificity Time Factors |
| Chemical | |
Reg. No./Substance:
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0/RNA Probes; 0/RNA, Ribosomal, 16S |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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