| Real-time Cell Analysis of Human Cancer Cell Lines after Chemotherapy with Functionalized Magnetic Nanoparticles. | |
| | |
MedLine Citation:
|
PMID: 22593476 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
BACKGROUND: Magnetic drug targeting is a new and innovative approach in cancer treatment. In order to avoid the adverse effects of chemotherapy, the therapeutic agent is linked to superparamagnetic nanoparticles which are injected into a tumour-supporting artery and is focused by an external magnetic field to the tumour region in order to provoke maximum local impact. Analysis of nanoparticles and chemotherapeutic substances in human cancer cell culture is necessary to provide respective information for in vivo applications. MATERIALS AND METHODS: The effect of pure mitoxantrone and mitoxantrone bound to nanoparticles was tested on human cancer cell lines using real-time cell analysis (RTCA) and lactate dehydrogenase (LDH) assays. RTCA was performed by impedance measuring. The impedance is expressed as the cell index (CI), which is a parameter of cell viability. RESULTS: RTCA showed that mitoxantrone when bound to nanoparticles was more toxic than the drug alone. The CI clearly decreased faster after adding the chemotherapeutic bound to nanoparticles than when adding the pure drug alone. However, in the first experiments, the particles themselves showed no toxicity at therapeutically relevant concentrations. These results were confirmed by LDH assays. CONCLUSION: The toxic effects of chemotherapeutic agents (e.g. mitoxantrone) on human cancer cell lines (e.g. MCF-7) can be enhanced if these drugs are bound to magnetic nanoparticles. These preliminary data show a dependency on the different application modes of RTCA. The results presented here are a first step for a better understanding of the effectiveness of magnetic drug targeting as a new and innovative cancer treatment. |
| | |
Authors:
|
Stephan Dürr; Stefan Lyer; Jenny Mann; Christina Janko; Rainer Tietze; Eveline Schreiber; Martin Herrmann; Christoph Alexiou |
Related Documents
:
|
22349096 - Stability of antibody-conjugated gold nanoparticles in the endolysosomal nanoenvironmen... 22894526 - In vitro investigation of efficient photodynamic therapy using a nonviral vector; hemag... 2048216 - The real role of risk assessment in cancer risk management. 22628656 - Metabolite profiling identifies a key role for glycine in rapid cancer cell proliferation. 16038026 - Nutritional factors and gastric cancer in zhoushan islands, china. 2596246 - High plasma levels of extrinsic pathway inhibitor and low levels of other coagulation i... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Anticancer research Volume: 32 ISSN: 1791-7530 ISO Abbreviation: Anticancer Res. Publication Date: 2012 May |
Date Detail:
|
Created Date: 2012-05-17 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8102988 Medline TA: Anticancer Res Country: Greece |
Other Details:
|
Languages: eng Pagination: 1983-9 Citation Subset: IM |
Affiliation:
|
Department of Otorhinolaryngology, Head and Neck Surgery, Section for Experimental Oncology and Nanomedicine (SEON), University Hospital, Waldstraße 1/Glückstraße 10, D-91054 Erlangen, Germany. c.alexiou@web.de. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Chromosomal Rearrangements in PLAG1 of Myoepithelial Salivary Gland Tumours.
Next Document: Vitamin D Status and Per-oral Vitamin D Supplementation in Patients Suffering from Chronic Pancreati...