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Real-Time Dynamics of RNA Polymerase II Clustering in Live Human Cells.
MedLine Citation:
PMID:  23828889     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Transcription is reported to be spatially compartmentalized in nuclear transcription factories with clusters of RNA Polymerase II (Pol II). However, little is known about when these foci assemble or their relative stability. We developed a quantitative single-cell approach to characterize protein spatiotemporal organization, with single-molecule sensitivity in live eukaryotic cells. We observed that Pol II clusters form transiently, with an average lifetime of 5.1 (± 0.4) seconds, refuting their notion as statically assembled substructures. Stimuli affecting transcription yielded orders-of-magnitude changes in the dynamics of Pol II clusters, which implies that clustering is regulated and plays a role in the cells ability to effect rapid response to external signals. Our results suggest that transient crowding of enzymes may aid in rate-limiting steps of gene regulation.
Authors:
Ibrahim I Cisse; Ignacio Izeddin; Sebastien Z Causse; Lydia Boudarene; Adrien Senecal; Leila Muresan; Claire Dugast-Darzacq; Bassam Hajj; Maxime Dahan; Xavier Darzacq
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-7-4
Journal Detail:
Title:  Science (New York, N.Y.)     Volume:  -     ISSN:  1095-9203     ISO Abbreviation:  Science     Publication Date:  2013 Jul 
Date Detail:
Created Date:  2013-7-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0404511     Medline TA:  Science     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Functional Imaging of Transcription, CNRS UMR8197, Ecole Normale Supérieure, Institut de Biologie de l'ENS, IBENS, Paris, 75005 France.
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