| Reagent for introducing pyrene residues in oligonucleotides. | |
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MedLine Citation:
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PMID: 1463786 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A novel pyrenyl-containing phosphoramidite reagent, N-[4-(1-pyrenyl)butyryl]-O1-(4,4'-dimethoxytrityl)-O2- [(diisopropylamino)(2-cyanoethoxy)phosphino]-3-amino-1 ,2-propanediol (5), has been synthesized from 4-(1-pyrenyl)butanoic acid in four steps with the 52% overall yield and used to incorporate pyrene residue(s) into oligonucleotides. Oligonucleotides 6 and 7, bearing one or two pyrenes at the 5'-terminus, have been prepared by means of that reagent, characterized with fluorescence spectra, and successfully used as primers in a polymerase chain reaction. |
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Authors:
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V A Korshun; N B Pestov; K R Birikh; Y A Berlin |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Bioconjugate chemistry Volume: 3 ISSN: 1043-1802 ISO Abbreviation: Bioconjug. Chem. Publication Date: 1992 Nov-Dec |
Date Detail:
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Created Date: 1993-01-19 Completed Date: 1993-01-19 Revised Date: 2000-12-18 |
Medline Journal Info:
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Nlm Unique ID: 9010319 Medline TA: Bioconjug Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 559-62 Citation Subset: IM |
Affiliation:
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M. M. Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Electrophoresis, Polyacrylamide Gel Indicators and Reagents* Molecular Sequence Data Oligonucleotides / chemistry* Polymerase Chain Reaction Pyrenes / chemistry* Spectrometry, Fluorescence |
| Chemical | |
Reg. No./Substance:
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0/Indicators and Reagents; 0/Oligonucleotides; 0/Pyrenes; 143039-35-4/N-(4-(1-pyrenyl)butyryl)-O(1)-(4,4'-dimethoxytrityl)-O(2)-((diisopropylamino)(2-cyanoethoxy)phosphino)-3-amino-1,2-propanediol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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