Document Detail

Readily available porcine aortic valve matrices for use in tissue valve engineering. Is cryopreservation an option?
MedLine Citation:
PMID:  16908013     Owner:  NLM     Status:  MEDLINE    
The clinical use of acellular biological valves as scaffolds in tissue valve engineering would require them to be readily available. This study examines the feasibility of cryopreserving porcine aortic valve matrices for use in tissue valve engineering. Matrices prepared using an enzymatic-detergent decellularization protocol were examined before and after cryopreservation. The biochemical status of tissues were evaluated by collagen and uronic acid (proteoglycan) determination and their mechanical properties were determined using a burst test. The histological and ultrastructural properties were evaluated by light and electron microscopy. Cryopreservation did not significantly affect the collagen and uronic acid content of aortic leaflet matrices. Histological and ultrastructural sections, however, confirmed extensive disruption of the extracellular collagen matrix and inter-fibrillar proteoglycan associations following cryopreservation. Although neither the breakage force nor the maximum force at failure was significantly different in matrices before and after cryopreservation, the strain observed in matrices was significantly higher after cryopreservation. To our knowledge this is the first study to investigate the effects of cryopreservation on aortic valve matrices. Cryopreservation did not significantly alter the biochemical properties of porcine aortic valve matrices. Nevertheless, cryopreservation had significant adverse effects on the structural and mechanical properties of matrices. Cryopreserved matrices showed significantly higher strain when stressed compared to non cryopreserved matrices. While, theoretically matrices are only expected to be functional for a limited time until regenerated in vivo, further mechanical testing is necessary to evaluate the effects of these changes on the durability of porcine aortic valve matrices for use in tissue valve engineering.
Kishan Narine; Erik Claeys Ing; Maria Cornelissen; Filip Desomer; Hilde Beele; Lieva Vanlangenhove; Stefaan De Smet; Guido Van Nooten
Related Documents :
1112703 - The late fate of autologous fascia lata valve grafts in the aortic position.
24796903 - Endoleaks after evar and tevar: indications for treatment and techniques.
8733673 - Oesophageal rupture in the course of conservative treatment of bleeding oesophageal var...
Publication Detail:
Type:  Journal Article     Date:  2006-08-14
Journal Detail:
Title:  Cryobiology     Volume:  53     ISSN:  0011-2240     ISO Abbreviation:  Cryobiology     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-09-18     Completed Date:  2007-01-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  169-81     Citation Subset:  IM    
Department of Cardiac Surgery K12 5Ve, The University Hospital Ghent, De Pintelaan 185, 9000 Ghent, Belgium.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Aorta / metabolism*
Aortic Valve / metabolism*,  pathology*
Collagen / chemistry
Cryopreservation / methods*
Cryoprotective Agents / pharmacology*
Detergents / pharmacology
Enzymes / chemistry
Extracellular Matrix / metabolism
Heart Valve Prosthesis
Proteoglycans / chemistry
Stress, Mechanical
Tissue Engineering / methods*
Uronic Acids / chemistry
Reg. No./Substance:
0/Cryoprotective Agents; 0/Detergents; 0/Enzymes; 0/Proteoglycans; 0/Uronic Acids; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Quantitative evaluation of monocyte transmigration into the brain following chemical opening of the ...
Next Document:  The preantral granulosa cell to cumulus cell transition in the mouse ovary: development of competenc...