| Readily available porcine aortic valve matrices for use in tissue valve engineering. Is cryopreservation an option? | |
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MedLine Citation:
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PMID: 16908013 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The clinical use of acellular biological valves as scaffolds in tissue valve engineering would require them to be readily available. This study examines the feasibility of cryopreserving porcine aortic valve matrices for use in tissue valve engineering. Matrices prepared using an enzymatic-detergent decellularization protocol were examined before and after cryopreservation. The biochemical status of tissues were evaluated by collagen and uronic acid (proteoglycan) determination and their mechanical properties were determined using a burst test. The histological and ultrastructural properties were evaluated by light and electron microscopy. Cryopreservation did not significantly affect the collagen and uronic acid content of aortic leaflet matrices. Histological and ultrastructural sections, however, confirmed extensive disruption of the extracellular collagen matrix and inter-fibrillar proteoglycan associations following cryopreservation. Although neither the breakage force nor the maximum force at failure was significantly different in matrices before and after cryopreservation, the strain observed in matrices was significantly higher after cryopreservation. To our knowledge this is the first study to investigate the effects of cryopreservation on aortic valve matrices. Cryopreservation did not significantly alter the biochemical properties of porcine aortic valve matrices. Nevertheless, cryopreservation had significant adverse effects on the structural and mechanical properties of matrices. Cryopreserved matrices showed significantly higher strain when stressed compared to non cryopreserved matrices. While, theoretically matrices are only expected to be functional for a limited time until regenerated in vivo, further mechanical testing is necessary to evaluate the effects of these changes on the durability of porcine aortic valve matrices for use in tissue valve engineering. |
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Authors:
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Kishan Narine; Erik Claeys Ing; Maria Cornelissen; Filip Desomer; Hilde Beele; Lieva Vanlangenhove; Stefaan De Smet; Guido Van Nooten |
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Publication Detail:
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Type: Journal Article Date: 2006-08-14 |
Journal Detail:
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Title: Cryobiology Volume: 53 ISSN: 0011-2240 ISO Abbreviation: Cryobiology Publication Date: 2006 Oct |
Date Detail:
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Created Date: 2006-09-18 Completed Date: 2007-01-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0006252 Medline TA: Cryobiology Country: United States |
Other Details:
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Languages: eng Pagination: 169-81 Citation Subset: IM |
Affiliation:
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Department of Cardiac Surgery K12 5Ve, The University Hospital Ghent, De Pintelaan 185, 9000 Ghent, Belgium. kishan.narine@UGent.be |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aorta / metabolism* Aortic Valve / metabolism*, pathology* Collagen / chemistry Cryopreservation / methods* Cryoprotective Agents / pharmacology* Detergents / pharmacology Enzymes / chemistry Extracellular Matrix / metabolism Heart Valve Prosthesis Proteoglycans / chemistry Stress, Mechanical Swine Tissue Engineering / methods* Uronic Acids / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Cryoprotective Agents; 0/Detergents; 0/Enzymes; 0/Proteoglycans; 0/Uronic Acids; 9007-34-5/Collagen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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