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Reactivity of human convalescent sera with influenza virus HA protein mutants at antigenic site A.
MedLine Citation:
PMID:  22309642     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
It is not well understood how the antibodies of individual convalescent human serum bind to each amino acid residue at the antigenic sites of hemagglutinin (HA) of influenza viruses, and how the antigenic drift strains of influenza viruses are selected by the human sera. In our previous study we indicated that the convalescent human sera, following A/Kamata/14/91(Ka/91)-like (H3N2) virus infection, bind to antigenic site A of Ka/91 HA, by a binding assay with a chimeric HA between Ka/91 and A/Aichi/2/68. Here we show how those human sera recognized each amino acid residue at antigenic site A, using the chimeric HAs possessing single amino acid substitutions at site A. The results showed; 1) there was a difference from person to person in the capacity of the human sera to recognize amino acid substitutions at site A, and 2) some amino acid substitutions caused all convalescent human sera to lose their binding capacity. Among them certain amino acid substitutions might be selected by chance for the successive antigenic drift. Phylogenetic analysis of the drift strains of Ka/91 showed amino acid substitutions at positions 133, 135 and 145 were on the main stream of the phylogenetic tree. Indeed, one of them was not recognized by all the investigated convalescent sera.
Authors:
Eri Nobusawa; Katsumi Omagari; Setsuko Nakajima; Katsuhisa Nakajima
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-2-7
Journal Detail:
Title:  Microbiology and immunology     Volume:  -     ISSN:  1348-0421     ISO Abbreviation:  -     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-2-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703966     Medline TA:  Microbiol Immunol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 The Societies and Blackwell Publishing Asia Pty Ltd.
Affiliation:
Department of Virology, Nagoya City University Graduate School of Medical Science, Nagoya City, Aichi, Japan.
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