Document Detail

Reactivity of creatinine with alkaline 3,5-dinitrobenzoate: a new fluorescent kidney function test.
MedLine Citation:
PMID:  7628067     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: To develop a new quantitative fluorometric assay for creatinine with enhanced specificity and inherent fluorescence sensitivity. DESIGN AND METHODS: Creatinine is reacted with 3,5-dinitrobenzoate under alkaline reaction conditions to produce a fluorophore. Alternatively, 3,5-dinitrobenzoyl chloride or methyl-3,5-dinitrobenzoate may be employed as reagents. Fluorophore development was evaluated for Group 1A bases under aqueous and mixed solvent conditions. RESULTS: The chemical reaction between creatinine and 3,5-dinitrobenzoate performed under highly alkaline conditions produced a fluorescent product with excitation and emission maxima near 410 and 475 nm, respectively. The reaction proceeds rapidly in the presence of group IA bases under both aqueous and mixed solvent conditions, in particular with water/dimethylsulfoxide and water/1,4-butanediol solvent systems containing 1.0 M LiOH. A linear calibration curve was obtained when the fluorescence intensity measured in microamperes was plotted versus creatinine concentration in the range of 5-50 mumol/L. The detection limit for creatinine was well below 1 mumol/L. CONCLUSIONS: A simple, sensitive and highly specific fluorometric method for the determination creatinine has been developed.
K G Blass
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical biochemistry     Volume:  28     ISSN:  0009-9120     ISO Abbreviation:  Clin. Biochem.     Publication Date:  1995 Apr 
Date Detail:
Created Date:  1995-09-05     Completed Date:  1995-09-05     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0133660     Medline TA:  Clin Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  107-11     Citation Subset:  IM    
Department of Chemistry, University of Regina, Saskatchewan, Canada.
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MeSH Terms
Creatinine / chemistry*
Kidney Function Tests*
Linear Models
Molecular Structure
Nitrobenzoates / chemistry*
Sensitivity and Specificity
Reg. No./Substance:
0/Alkalies; 0/Nitrobenzoates; 60-27-5/Creatinine; 99-34-3/3,5-dinitrobenzoic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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